engleski

Characterization of dispersed alpha-satellite DNA elements in human genome

Satellite DNAs are tandemly repeated sequences present in long uninterupted arrays in genetically silenced heterochromatic regions. Genome sequencing projects revealed the presence and the distribution of repetitive satellite DNA in the vicinity of genes, or in other words, the dispersion of satellite DNA elements across the euchromatic portion of genome. The presence of functional elements such as promoters and transcription factors binding sites within a number of satelites led to assumption that satellite DNAs may act as gene expression cis-regulatory elements. The main goal of this research is to determine the abundance and the distribution of dispersed elements of alpha satellite DNA in human genome and to propose them as genetic polymorphisms in the population, as well as to investigate the potential regulatory role of those elements by DNA methylation analysis before and after the heat-shock treatment.

alpha satellite DNA ; cis-regulatory elements ; insertion polymorphism ; adaptation to heat stress

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