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Pregled bibliografske jedinice broj: 982605

Anti-cancer organoruthenium(II) complexes and their interactions with cysteine and its analogues. A mass-spectrometric study


Briš, Anamarija; Jašík, Juraj; Turel, Iztok; Roithová, Jana
Anti-cancer organoruthenium(II) complexes and their interactions with cysteine and its analogues. A mass-spectrometric study // Dalton Transactions, 2019 (2019), 48; 1-9 doi:10.1039/c8dt04350g (međunarodna recenzija, članak, znanstveni)


Naslov
Anti-cancer organoruthenium(II) complexes and their interactions with cysteine and its analogues. A mass-spectrometric study

Autori
Briš, Anamarija ; Jašík, Juraj ; Turel, Iztok ; Roithová, Jana

Izvornik
Dalton Transactions (1477-9226) 2019 (2019), 48; 1-9

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Amino acids ; anti-cancer activity ; IRPD spectroscopy ; mass spectrometry ; ruthenium

Sažetak
The ruthenium complexes [Ru(CYM)(p-Cl-dkt)(Cl)] (1), [Ru(CYM)( pta)(p-Cl-dkt)]PF6 (2), and [Ru(CYM)(pta)Cl2] (3, RAPTA-C) (CYM = para- cymene, p-Cl-dkt = 1-(4-chlorophenyl)-4, 4, 4- trifluorobutane-1, 3-dione, pta = 1, 3, 5-triaza- 7-phosphaadamantane) are biologically active and show anti-cancer activities, albeit with different mechanisms. To further understand these mechanisms, we compared their speciation in aqueous solutions with an amino acid (cysteine), with an amino acid derivative (N- acetylcysteine) and with a tripeptide (glutathione) by Mass Spectrometry (MS). Here, we show that all ruthenium complexes have high selectivity for cysteine and cysteine-derived molecules. On one hand, [Ru(CYM)(p-Cl-dkt)(Cl)] undergoes solvolysis in water and forms [Ru2(CYM)2(OH)3]+. Subsequently, all hydroxyl anions are exchanged by deprotonated cysteine. Infrared Photodissociation Spectroscopy (IRPD) showed that cysteine binds to the ruthenium atoms via the deprotonated thiol group and that sulfur bridges the ruthenium centers. On the other hand, the pta-bearing complexes remain monometallic and undergo only slow Cl or p-Cl- dkt exchange by deprotonated cysteine. Therefore, the pta ligand protects the ruthenium complexes from ligand exchange with water and from the formation of biruthenium clusters, possibly explaining why the mechanism of pta-bearing ruthenium complexes is not based on ROS production but on their reactivity as monometallic complexes.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Ustanove
Institut "Ruđer Bošković", Zagreb

Autor s matičnim brojem:
Anamarija Briš, (317583)

Citiraj ovu publikaciju

Briš, Anamarija; Jašík, Juraj; Turel, Iztok; Roithová, Jana
Anti-cancer organoruthenium(II) complexes and their interactions with cysteine and its analogues. A mass-spectrometric study // Dalton Transactions, 2019 (2019), 48; 1-9 doi:10.1039/c8dt04350g (međunarodna recenzija, članak, znanstveni)
Briš, A., Jašík, J., Turel, I. & Roithová, J. (2019) Anti-cancer organoruthenium(II) complexes and their interactions with cysteine and its analogues. A mass-spectrometric study. Dalton Transactions, 2019 (48), 1-9 doi:10.1039/c8dt04350g.
@article{article, year = {2019}, pages = {1-9}, DOI = {10.1039/c8dt04350g}, keywords = {amino acids, anti-cancer activity, IRPD spectroscopy, mass spectrometry, ruthenium}, journal = {Dalton Transactions}, doi = {10.1039/c8dt04350g}, volume = {2019}, number = {48}, issn = {1477-9226}, title = {Anti-cancer organoruthenium(II) complexes and their interactions with cysteine and its analogues. A mass-spectrometric study}, keyword = {amino acids, anti-cancer activity, IRPD spectroscopy, mass spectrometry, ruthenium} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
  • Scopus
  • MEDLINE


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