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Pregled bibliografske jedinice broj: 982270

IQGAP-related protein IqgC suppresses Ras signaling during large-scale endocytosis


Marinović, Maja; Mijanović, Lucija; Šoštar, Marko; Vizovišek, Matej; Junemann, Alexander; Fonović, Marko; Turk, Boris; Weber, Igor; Faix, Jan; Filić, Vedrana
IQGAP-related protein IqgC suppresses Ras signaling during large-scale endocytosis // Proceedings of the National Academy of Sciences of the United States of America, 116 (2019), 4; 1289-1298 doi:10.1073/pnas.1810268116 (međunarodna recenzija, članak, znanstveni)


Naslov
IQGAP-related protein IqgC suppresses Ras signaling during large-scale endocytosis

Autori
Marinović, Maja ; Mijanović, Lucija ; Šoštar, Marko ; Vizovišek, Matej ; Junemann, Alexander ; Fonović, Marko ; Turk, Boris ; Weber, Igor ; Faix, Jan ; Filić, Vedrana

Izvornik
Proceedings of the National Academy of Sciences of the United States of America (0027-8424) 116 (2019), 4; 1289-1298

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
IqgC ; RasGAP ; Ras ; macropinocytosis ; phagocytosis

Sažetak
Macropinocytosis and phagocytosis are evolutionarily conserved forms of bulk endocytosis used by cells to ingest large volumes of fluid and solid particles, respectively. Both processes are regulated by Ras signaling, which is precisely controlled by mechanisms involving Ras GTPase activating proteins (RasGAPs) responsible for terminating Ras activity on early endosomes. While regulation of Ras signaling during large-scale endocytosis in WT Dictyostelium has been, for the most part, attributed to the Dictyostelium ortholog of human RasGAP NF1, in commonly used axenic laboratory strains, this gene is mutated and inactive. Moreover, none of the RasGAPs characterized so far have been implicated in the regulation of Ras signaling in large-scale endocytosis in axenic strains. In this study, we establish, using biochemical approaches and complementation assays in live cells, that Dictyostelium IQGAP-related protein IqgC interacts with active RasG and exhibits RasGAP activity toward this GTPase. Analyses of iqgC− and IqgC-overexpressing cells further revealed participation of this GAP in the regulation of both types of large-scale endocytosis and in cytokinesis. Moreover, given the localization of IqgC to phagosomes and, most prominently, to macropinosomes, we propose IqgC acting as a RasG-specific GAP in large-scale endocytosis. The data presented here functionally distinguish IqgC from other members of the Dictyostelium IQGAP family and call for repositioning of this genuine RasGAP outside of the IQGAP group.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
EK-FP7-316289 (EK - FP7-REGPOT-2012-2013-1)

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE
  • EconLit


Uključenost u ostale bibliografske baze podataka:


  • PsycLit (Psychological Abstracts)
  • CABI
  • EBSCOhost
  • Gale
  • Journal Watch
  • JSTOR
  • OCLC
  • Portico
  • ProQuest
  • Psychological Abstracts
  • RePEc


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