engleski

VOLATILE COMPOUNDS OF PHACELIA (Phacelia tanacetifolia Benth.) HONEY BEFORE AND AFTER HEATING

Many compounds of biological and botanical origin such as volatile substances are unstable. In honey the structures of volatile substances can be transformered during maturation, storage and heating. In this paper the influence of phacelia honey heating on the composition and content of volatile compounds is investigated. The honey sample were heated at 150 oC (5 min.) without addition of water to notice thermal artifacts. Volatile compounds were extracted with dichloromethane using ultrasound assisted solvent extraction (USE) at room temperature. The extracts were analyzed by gas chromatography-mass spectrometry (GC-MS). Phacelia tanacetifolia Benth. honey biomarker syringaldehyde proved to be stable throughout heating procedure up to 150 oC. The dominant compounds identified in dichloromethane extract obtained after heating of honey at 150 oC were thermal artefacts 5- hydroxymethylfurfural and pyran derivative 2, 3- dihydro-3, 5-dihydroxy-6-methyl-4H-pyran-4-one.

phacelia honey heating, USE, GC-MS analysis, volatile compounds, thermal artefacts

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