engleski

Protein hydrolysates from hempseed oil-cake as cell culture media supplement

Animal cells are a complex expression system applied in wide span of biotech and biomedical techniques. Cell growth supporting microenvironment is largely defined by nutrient composition of culture media. Content of the proteins as much as their origin have a key role in preparation of culture media. Fetal bovine serum is still used as a major protein source, despite its compositional inconsistency and safety related risks. To overcome these problems, cell culture experts developed serum- free media where protein hydrolysates, preferably of plant origin, replace serum proteins. Among the most promising substances for the hydrolysate production are protein rich byproducts of kitchen oil industry called oil-cakes or oil-meals. Oil-cakes of hempseed contain about 30% proteins, and as such can be processed into nutrient media supplement for animal cell cultivation. The main objective of the proposed study is to determine the effects of hempseed oil-cake protein hydrolysates on growth and productivity of IgG producing cell lines. The protein hydrolysis was carried out with commercial microbial proteases. Our preliminary results indicated potential application of the hydrolysates as beneficial culture media component.

animal cells, hydrolysates, serum-free media

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