engleski

Current status of Pepino mosaic virus (PepMV) in Croatia

Pepino mosaic virus (PepMV) was first reported on greenhouse tomato (Solanum lycopersicum) in the Netherlands in 1999. Since then this viral disease has been established in the tomato industry worldwide and become a significant problem. PepMV is an EPPO alert list pest. In order to verify the presence of PepMV in tomato cultivars and to enable enforcement of European Union legislation, an extensive survey among Croatian tomato growers was conducted in 2004. During a six years of monitoring, 1065 samples were tested: 1050 samples from commercial domestic greenhouse tomato production, 13 samples from imported seedlings and two from imported tomato fruits. Samples were analysed by enzyme-linked immunosorbent assay, DAS-ELISA (using the Loewe antisera) and DASI-ELISA (using the Neogen antisera) respectively. The extension with IC-RT-PCR was introduced in 2007 by the use of PepMV specific antibodies (Loewe) and oligonucleotide primers. The results obtained by IC-RT-PCR analysis in 2007 were in complete agreement with both ELISA methods (DAS, DASI) showing that one tomato fruit sample (imported from Italy) was found to be infected with PepMV. These results confirmed the absence of PepMV in greenhouse tomato production in Croatia. Therefore, it becomes extremely important to use certificated seed, apply sanitary measures and conduct regular inspection monitoring in the future.

tomato, greenhouse production, DAS-ELISA, DASI-ELISA, IC-RT-PCR

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