engleski

Biodiversity of indigenous rhizobia nodulating Phaseolus vulgaris in Croatia

Common bean production in Croatia is limited to small-scale farmers and generally neglected despite favourable environmental conditions. It is assumed that indigenous rhizobial strains with hidden potential for efficient nitrogen fixation are present in Croatian soils. The main aim of the present study was to determine genetic diversity of indigenous common bean rhizobia isolated from different field sites in the Northwestern Croatia. Identification of isolates was performed by MALDI-TOF MS, RAPD fingerprinting and rrs, recA, atpD and nodC gene sequencing. The results obtained with MALDI-TOF MS methodology show that the strains from this study matched with different species from the Rhizobium leguminosarum phylogenetic group with score values higher than 2.0. The analysis of the core genes rrs, recA and atpD showed that most of strains isolated from common bean nodules in Croatia belong to the species R. leguminosarum and R. hidalgonense. Two strains were identified as R. pisi and several strains formed lineages phylogenetically divergent from all species currenlty encompassed in the Rhizobium leguminosarum phylogenetic group.

Rhizobium, Phaseolus vulgaris, gene sequencing, MALDI-TOF

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