engleski

Appearance of polar and non-polar cytoskeleton filaments in neuronal cells after GSM modulated RF exposure

The study was carried out on the purpose to assess the reaction of polar and non-polar cytoskeleton filaments after Global System of Mobile (GSM) modulated radiofrequency (RF) radiation. Culture of neuronal cell line SH-SY5Y was exposed to GSM modulated RF radiation frequency of 915 MHz, electric field strength of 10, 20 and 30 V/m. Average specific absorption rate (SAR) was calculated at 0.23, 0.8 and 1.6 W/kg. Cell exposure treatment lasted for 1, 2, and 3 hours. Negative- and positive control cell samples were matched with experimental cells samples. Gigahertz Transversal Electromagnetic Mode chamber (GTEM), generator, power amplifier and signal modulator were the parts of the radiation exposure set-up. To define polar actin microfilament structure, rhodamine-phalloidin fluorescent technique was used. For determination of nonpolar vimentin filaments, indirect immunocytochemistry method was used. Five hundred cells were examined under the fluorescent microscope to get insight into the structure of actin and vimentin filaments. In comparison with negative control cell samples, polar actin microfilaments were significantly altered after three hour of exposure to 10, 20 and 30 V/m electric field strength. Opposing to actin, non-polar vimentin filaments did not respond to applied radiation in any other way, i.e., the structure remained unchanged throughout the course of experiment. Contrary to non-polar cytoskeleton vimentin filaments, structures of polar actin were significantly altered. Alteration of actin microfilament structure was rather time dependent then dose dependent. The polar structure with internal electromagnetic field within the RF range can be affected by external GSM modulated RF radiation frequency of 915 MHz.

in vitro: RF/Microwaves

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