engleski

Cytotoxic and intracellular biomarkers of response to non-ionizing radiation evaluated in neuronal cells

The aim of this study was to evaluate whether low-level, modulated nonizonizing radiation (NIR) irradiation frequentation of 915 MHz influences the cell viability, proliferation and intracellular microtubular structures of SH-SY5Y neuroblastoma cells. Modulated NIR field was generated inside a Gigahertz Transversal Electromagnetic Mode cell (GTEM-cell) with Anritsu spectrum analyzer with tracking generator and amplifier. The electric field strength was 10, 20 and 30 V/cm and the average specific absorption rate (SAR) was calculated to be 0.12, 0.4 and 0.8 W/kg. Cell samples were cultivated in a temperature controlled atmosphere at 37 °C with 5% CO2 and high humidity. Previously prepared cells were exposed to a 915 MHz continuous wave frequency field for 3 hours. Cell viability was determined by Trypan blue method, whereas cell proliferation was determined by MTT test. The structure of microtubule proteins has been determined using the immunocytochemical method. Negative- and positive cell controls were included into the experimental procedure. Results showed that modulated NIR did not have impact on celullar viability and proliferation. In comparison with control cells, the microtubule structure altered after 3 hours of 30 V/m irradiation. It seems that the modulated 915 MHz, low-level NIR radiation affects microtubule proteins without influencing cell proliferation and viability

cell proliferation: cell viabillity: microtubule protein: modulated nonionizing radiation

nije evidentirano