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Lipid Membranes as a Target for Reactive Aldehyde Action

Reactive aldehydes, such as 4-hydroxynonenal (4-HNE) and 4-oxononenal (4-ONE), are peroxidation products of polyunsaturated fatty acids formed in cellular membranes during oxidative stress. Recently, we demonstrated that their interaction with phosphoethanolamine in the cell membrane alters the function of membrane carriers, such as mitochondrial uncoupling proteins, valinomycin or CCCP (1, 2). In this work, we observed that (i) 4-HNE increased the total membrane conductance, Gm, in mixed DOPE:DOPC bilayers in contrast to DOPC bilayers, (ii) the Gm increase depended on the cation concentration in buffer solution (iii) 4-ONE did not affect Gm at any lipid composition. Together with MD simulations the results offer an explanation as to why 4-HNE and 4-ONE have a different effect on Gm and how a small, but important difference in the chemical structure of 4-ONE compared to 4-HNE lead to different properties of biological membranes during oxidative stress.

lipid bilayer ; reactive aldehydes ; conductivity ; molecular dynamics

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