engleski

Eskape pathogens in Croatian soil

The term “ESKAPE” pathogens includes a group of bacteria connected to human infections that can escape the biocidal action of antibiotics. Bacterial resistance to carbapenems has received special attention, because carbapenems are used as a last-resort antibiotics to treat infections caused by antibiotic-resistant bacteria. Carbapenem-resistant Acinetobacter baumannii is positioned at the top of the World Health Organization priority list, for which new antibiotics are urgently needed. Little is known about the presence of this emerging human pathogen outside the hospital settings. Here we report the finding of three carbapenem-resistant isolates of A. baumannii in technosol at an illegal dumpsite in Croatia. Developed technosol at the edge of the dump was collected in October 2016. The A. baumannii were recovered from inoculated plates of CHROMagar Acinetobacter supplemented with 15 mg/L of cefsulodin sodium salt hydrate, after incubation at 42 °C/48 h. The identification of species was performed by molecular methods. The antibiotic susceptibility profile was interpreted according to the official criteria for clinical isolates. Three isolates of A. baumannii shared features with the widespread human clinical isolates: affiliation to the international clonal lineage IC1 (ST-231) or IC2 (ST-195), multi-drug resistance, carbapenem-resistance mediated by acquired blaOXA-72 and blaOXA-23 genes. This suggest the illegally disposed hospital waste as the most probable source of A. baumannii in technosol. Proper management and disposal of human solid waste is mandatory to prevent the spread of ESKAPE pathogens in nature.

Bacteria, antibiotic resistance, Acinetobacter baumannii, soil

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