Expression of proinflammatory cytokine interleukin-6 in tissue samples of human prostate obtained by needle biopsy. (CROSBI ID 258435)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Miličević, Nevenka ; Mrčela, Milanka ; Galić, Josip ; Marjanović, Ksenija
engleski
Expression of proinflammatory cytokine interleukin-6 in tissue samples of human prostate obtained by needle biopsy.
Interleukin-6 (IL-6) has been associated with the development of prostate cancer. The aim of the study was to clarify whether IL-6 expression in prostate tissue could be a useful marker in differentiation of prostate diseases in small foci by pathologist visual scoring. Archival paraffin-embedded specimens of benign prostate hyperplasia (BPH), high-grade prostatic intraepithelial neoplasia (PIN), prostatitis and prostate adenocarcinoma were studied by immunohistochemistry with a mouse monoclonal antibody IL-6 using the streptavidin-biotin method. Significantly, lower IL-6 immunoreactivity was observed in normal epithelial cells (p=0.000) and basal cells (p=0.000) in the samples of prostate adenocarcinoma in comparison to the samples with BPH, PIN and prostatitis. There was no significant difference in IL-6 expression in malignant and premalignant cells (p=0.814) as well as in stromal cells among the four diagnoses (p=0.22). IL-6 was expressed in normal epithelial cells, premalignant epithelial cells and malignant epithelial cells as well as in stromal cells. However, in our research IL-6 was of limited utility as a single marker for differential diagnosis of the prostate diseases in small foci needle biopsy by pathologist visual scoring. The standardization of immunohistochemical (IHC) staining protocol for IL-6 is required to determine IL-6 expression in order to avoid possible misinterpretation of the IHC results.
Immunohistochemical staining ; Interleukin-6 ; Needle biopsy ; Pathologist visual scoring ; Prostate disease
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Podaci o izdanju
211 (11)
2015.
865-870
objavljeno
0344-0338
1618-0631
10.1016/j.prp.2015.08.008
Povezanost rada
Kliničke medicinske znanosti