Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 976212

Toward Higher Sensitivity in Quantitative MALDI Imaging Mass Spectrometry of CNS Drugs Using a Nonpolar Matrix


Rzagalinski, Ignacy; Kovačević, Borislav; Hainz, Nadine; Meier, Carola; Tschernig, Thomas; Volmer, Dietrich A.
Toward Higher Sensitivity in Quantitative MALDI Imaging Mass Spectrometry of CNS Drugs Using a Nonpolar Matrix // Analytical Chemistry, 90 (2018), 21; 12592-12600 doi:10.1021/acs.analchem.8b02740 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 976212 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Toward Higher Sensitivity in Quantitative MALDI Imaging Mass Spectrometry of CNS Drugs Using a Nonpolar Matrix

Autori
Rzagalinski, Ignacy ; Kovačević, Borislav ; Hainz, Nadine ; Meier, Carola ; Tschernig, Thomas ; Volmer, Dietrich A.

Izvornik
Analytical Chemistry (0003-2700) 90 (2018), 21; 12592-12600

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DCTB ; 2-[(2E)-3-(4-tert-butylphenyl)-2-methylprop-2-enylidene]malononitrile ; mass spectrometry imaging ; MALDI ; FTICR ; CNS drugs ; xylazine

Sažetak
Tissue-specific ion suppression is an unavoidable matrix effect in MALDI mass spectrometry imaging (MSI), the negative impact of which on precision and accuracy in quantitative MALDI-MSI can be reduced to some extent by applying isotope internal standards for normalization and matrix- matched calibration routines. The detection sensitivity still suffers, however, often resulting in significant loss of signal for the investigated analytes. An MSI application considerably affected by this phenomenon is the quantitative spatial analysis of central nervous system (CNS) drugs. Most of these drugs are low molecular weight, lipophilic compounds, which exhibit inefficient desorption and ionization during MALDI using conventional polar acidic matrices (CHCA, DHB). Here, we present the application of the (2-[(2E)-3-(4-tert- butylphenyl)-2-methylprop-2- enylidene]malononitrile) matrix for high sensitivity imaging of CNS drugs in mouse brain sections. Since DCTB is usually described as electron-transfer matrix, we provide a rationale (i.e. computational calculations of gas-phase proton affinity and ionization energy) for an additional proton-transfer ionization mechanism with this matrix. Furthermore, we compare the extent of signal suppression for five different CNS drugs when employing DCTB versus CHCA matrices. The results showed that the signal suppression was not only several times lower with DCTB than with CHCA, but also depended on the specific tissue investigated. Finally, we present the application of DCTB and ultra-high resolution Fourier-transform ion cyclotron resonance mass spectrometry to quantitative MALDI imaging of the anesthetic drug xylazine in mouse brain sections based on a linear matrix-matched calibration curve. DCTB afforded up to 100-fold signal intensity improvement over CHCA when comparing representative single MSI pixels, and > 440-fold for the averaged mass spectrum of the adjacent tissue sections.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Borislav Kovačević (autor)

Citiraj ovu publikaciju

Rzagalinski, Ignacy; Kovačević, Borislav; Hainz, Nadine; Meier, Carola; Tschernig, Thomas; Volmer, Dietrich A.
Toward Higher Sensitivity in Quantitative MALDI Imaging Mass Spectrometry of CNS Drugs Using a Nonpolar Matrix // Analytical Chemistry, 90 (2018), 21; 12592-12600 doi:10.1021/acs.analchem.8b02740 (međunarodna recenzija, članak, znanstveni)
Rzagalinski, I., Kovačević, B., Hainz, N., Meier, C., Tschernig, T. & Volmer, D. (2018) Toward Higher Sensitivity in Quantitative MALDI Imaging Mass Spectrometry of CNS Drugs Using a Nonpolar Matrix. Analytical Chemistry, 90 (21), 12592-12600 doi:10.1021/acs.analchem.8b02740.
@article{article, year = {2018}, pages = {12592-12600}, DOI = {10.1021/acs.analchem.8b02740}, keywords = {DCTB, 2-[(2E)-3-(4-tert-butylphenyl)-2-methylprop-2-enylidene]malononitrile, mass spectrometry imaging, MALDI, FTICR, CNS drugs, xylazine}, journal = {Analytical Chemistry}, doi = {10.1021/acs.analchem.8b02740}, volume = {90}, number = {21}, issn = {0003-2700}, title = {Toward Higher Sensitivity in Quantitative MALDI Imaging Mass Spectrometry of CNS Drugs Using a Nonpolar Matrix}, keyword = {DCTB, 2-[(2E)-3-(4-tert-butylphenyl)-2-methylprop-2-enylidene]malononitrile, mass spectrometry imaging, MALDI, FTICR, CNS drugs, xylazine} }
@article{article, year = {2018}, pages = {12592-12600}, DOI = {10.1021/acs.analchem.8b02740}, keywords = {DCTB, 2-[(2E)-3-(4-tert-butylphenyl)-2-methylprop-2-enylidene]malononitrile, mass spectrometry imaging, MALDI, FTICR, CNS drugs, xylazine}, journal = {Analytical Chemistry}, doi = {10.1021/acs.analchem.8b02740}, volume = {90}, number = {21}, issn = {0003-2700}, title = {Toward Higher Sensitivity in Quantitative MALDI Imaging Mass Spectrometry of CNS Drugs Using a Nonpolar Matrix}, keyword = {DCTB, 2-[(2E)-3-(4-tert-butylphenyl)-2-methylprop-2-enylidene]malononitrile, mass spectrometry imaging, MALDI, FTICR, CNS drugs, xylazine} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
  • Scopus
  • MEDLINE


Citati





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font