Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades (CROSBI ID 257963)
Prilog u časopisu | izvorni znanstveni rad
Podaci o odgovornosti
Kafka, Anja ; Karin-Kujundžić, Valentina ; Šerman, Ljiljana ; Bukovac, Anja ; Njirić, Niko ; Jakovčević, Antonia ; Pećina-Šlaus, Nives
engleski
Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades
Aim: To identify the involvement of Secreted Frizzled Related Protein 1 (SFRP1) promoter hypermethylation in different malignancy grades of astrocytoma and assess its association with beta- catenin, lymphoid-enhancer factor 1, and T-cell factor 1. Methods: Twenty-six astrocytoma samples were collected from 2008-2015. Promoter hypermethylation was evaluated by methylation- specific polymerase-chain-reaction and protein expression by immunohistochemistry and stereological analysis. The staining intensity was scored by comparing immunoreactivity with normal tissue and by using 10% and 50% cut-offs. Results: SFRP1 promoter methylation was found in 32% of astrocytomas. The number of hypermethylated samples increased in higher astrocytoma grades and was the highest in glioblastoma (P = 0.042 compared to other astrocytoma grades). There was 45.8% of samples with the lack of or weak expression of SFRP1 protein and 29.2% with strong expression. Samples with methylated promoter expressed significantly less SFRP1 than samples with unmethylated promoter (P = 0.031). Beta- catenin expression levels were elevated. Yet, glioblastomas with unmethylated SFRP1 promoter had significantly less beta-catenin (P = 0.033). Strong expression of lymphoid-enhancer factor 1 was associated to higher astrocytoma grades (P = 0.006). Conclusion: SFRP1 gene was epigenetically silenced in glioblastomas when compared to low astrocytoma grades, which may suggest that the lack of its protein is involved in astrocytoma progression.
SFRP1 ; DNA methylation ; Wnt signaling ; astrocytic brain tumors ; LEF1 ; TCF1 ; beta-catenin
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o izdanju
59 (5)
2018.
213-223
objavljeno
0353-9504
1332-8166
10.3325/cmj.2018.59.213
Povezanost rada
Biologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje), Temeljne medicinske znanosti