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Pregled bibliografske jedinice broj: 973791

A new role of RecA protein in Escherichia coli?


Đermić, Edyta; Zahradka, Davor; Vujaklija, Dušica; Đermić, Damir
A new role of RecA protein in Escherichia coli? // 4th Congress of Croatian Geneticists with International Participation - Book of Abstracts / Šarčević, Hrvoje ; Ugarković, Đurđica ; Vujaklija, Dušica ; Svetec, Ivan Krešimir ; Svetec Miklenić, Marina (ur.).
Zagreb: Croatian Genetic Society, 2018. str. 44-44 (poster, recenziran, sažetak, znanstveni)


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Naslov
A new role of RecA protein in Escherichia coli?

Autori
Đermić, Edyta ; Zahradka, Davor ; Vujaklija, Dušica ; Đermić, Damir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
4th Congress of Croatian Geneticists with International Participation - Book of Abstracts / Šarčević, Hrvoje ; Ugarković, Đurđica ; Vujaklija, Dušica ; Svetec, Ivan Krešimir ; Svetec Miklenić, Marina - Zagreb : Croatian Genetic Society, 2018, 44-44

ISBN
978-953-57128-1-7

Skup
4th Congress of Croatian Geneticists with International Participation

Mjesto i datum
Krk, Hrvatska, 26-29.09.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Recenziran

Ključne riječi
Escherichia coli ; RecA protein ; DNA double-strand breaks ; DNA degradation

Sažetak
The ubiquitous and evolutionary conserved RecA protein binds to single-stranded DNA (ssDNA), producing a nucleoprotein filament. RecA has three known roles in E. coli: a) RecA nucleoprotein filament enables repair of DNA double-strand breaks (DSBs) and exchange of similar DNA molecules by promoting homologous recombination ; b) RecA nucleofilament facilitates autocleavage of LexA repressor, hence enabling induction of SOS response ; c) during SOS induction, RecA activates a mutagenic DNA polymerase V. Another role of RecA exists in E. coli, namely RecA inhibits DNA degradation. However, no relation of the latter RecA function to the three established ones has been explored previously. Here, we introduced DSBs (by gamma-irradiation) into E. coli chromosome labelled by [3H]thymidine and monitored its degradation. We have shown that RecA inhibition of DNA degradation depends on RecA’s concentration and its rate of association with ssDNA. Also, our results indicate that RecA suppression of DNA degradation is separable from the other three roles of RecA, thus suggesting it represents a novel RecA function in E. coli, namely regulation of DSB processing.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
HRZZ-IP-2013-11-2978 - Rekombinacija, popravak DNA i očuvanje integriteta genoma: novi putevi (RECNEWPATH) (Zahradka, Davor, HRZZ - 2013-11) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb,
Agronomski fakultet, Zagreb

Profili:

Avatar Url Edyta Đermić (autor)

Avatar Url Davor Zahradka (autor)

Avatar Url Damir Đermić (autor)

Avatar Url Dušica Vujaklija (autor)


Citiraj ovu publikaciju

Đermić, Edyta; Zahradka, Davor; Vujaklija, Dušica; Đermić, Damir
A new role of RecA protein in Escherichia coli? // 4th Congress of Croatian Geneticists with International Participation - Book of Abstracts / Šarčević, Hrvoje ; Ugarković, Đurđica ; Vujaklija, Dušica ; Svetec, Ivan Krešimir ; Svetec Miklenić, Marina (ur.).
Zagreb: Croatian Genetic Society, 2018. str. 44-44 (poster, recenziran, sažetak, znanstveni)
Đermić, E., Zahradka, D., Vujaklija, D. & Đermić, D. (2018) A new role of RecA protein in Escherichia coli?. U: Šarčević, H., Ugarković, Đ., Vujaklija, D., Svetec, I. & Svetec Miklenić, M. (ur.)4th Congress of Croatian Geneticists with International Participation - Book of Abstracts.
@article{article, year = {2018}, pages = {44-44}, keywords = {Escherichia coli, RecA protein, DNA double-strand breaks, DNA degradation}, isbn = {978-953-57128-1-7}, title = {A new role of RecA protein in Escherichia coli?}, keyword = {Escherichia coli, RecA protein, DNA double-strand breaks, DNA degradation}, publisher = {Croatian Genetic Society}, publisherplace = {Krk, Hrvatska} }
@article{article, year = {2018}, pages = {44-44}, keywords = {Escherichia coli, RecA protein, DNA double-strand breaks, DNA degradation}, isbn = {978-953-57128-1-7}, title = {A new role of RecA protein in Escherichia coli?}, keyword = {Escherichia coli, RecA protein, DNA double-strand breaks, DNA degradation}, publisher = {Croatian Genetic Society}, publisherplace = {Krk, Hrvatska} }




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