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BPM1 protein stability in Arabidopsis thaliana is dependent on environmental temperature

MATH-BTB proteins are comprised of 2 domains, MATH (Meprin and TRAF Homology) and BTB (Bric- A-Brac, Tramtrack, Broad Complex). They have been shown to interact with Cul3-based E3 ligase complex which promotes proteasomal degradation of target proteins. The Arabidopsis thaliana genome encodes 6 MATH-BTB genes (AtBPM1-6). Through interaction with members of at least three families of transcription factors (R2R3 MYB family, ethylene response factor/Apetala2 and class I homeobox-leucine zipper transcription factors), BPMs are involved in plant flowering, seed development and abiotic stress response. According to recent findings, BPM proteins are responsible for degradation of transcription factor DREB2A involved in heat- and drought-stress response. To examine physiological roles of BPM1 in heat stress reponse, A. thaliana plants were transformed to obtain transgenic plants overexpressing GFP-tagged BPM1. Homozygous seedlings were exposed to elevated temperature and heat stress followed by whole soluble protein extraction. Anti-GFP monoclonal antibody was used for immunodetection of transgenic GFP-BPM1 protein. In control conditions, recombinant BPM1 exhibited low stability despite high overexpression rates. However, elevated temperatures and heat stress caused significant stabilization of transgenic BPM1. This result could indicate the physiological role of AtBPM1 in response to heat stress in Arabidopsis.

Arabidopsis, BPM1, heat stress, immunodetection

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