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Pregled bibliografske jedinice broj: 970134

AtBPM1 protein interacts with components of RNA- directed DNA methylation


Jagić, Mateja; Škiljaica, Andreja; Bauer, Nataša; Leljak-Levanić, Dunja
AtBPM1 protein interacts with components of RNA- directed DNA methylation // Final Programme & Book of Abstracts: FEBS3+ From molecules to living systems / Szüts, Dávis ; Buday, László (ur.).
Veszprem, Mađarska: OOK-Press Ltd, 2018. str. 124-124 (poster, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 970134 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
AtBPM1 protein interacts with components of RNA- directed DNA methylation

Autori
Jagić, Mateja ; Škiljaica, Andreja ; Bauer, Nataša ; Leljak-Levanić, Dunja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Final Programme & Book of Abstracts: FEBS3+ From molecules to living systems / Szüts, Dávis ; Buday, László - Veszprem, Mađarska : OOK-Press Ltd, 2018, 124-124

ISBN
978-615-5270-47-5

Skup
FEBS3+ From molecules to living systems

Mjesto i datum
Siófok, Mađarska, 02-05.09.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
MATH-BTB, Arabidopsis thaliana, protein-protein interactions, RNA-directed DNA methylation

Sažetak
AtBPM1 belongs to the small family of Arabidopsis MATH and BTB domain proteins. A family of MATH-BTB proteins is known to act as substrate-specific adaptors of CUL3-based E3 ligases in the ubiquitin proteasome pathway. While BTB domain binds a CUL3 scaffold protein, the less conserved MATH domain targets a highly diverse collection of substrate proteins to promote their ubiquitination and subsequent degradation. Tandem affinity purification and yeast two hybrid assay revealed interaction of BPM1 with DMS3 and RDM1, crucial components of RNA-directed DNA methylation pathway (RdDM). Fluorescently tagged BPM1 and its truncated version with deletion of MATH or BTB domain showed that both domains are essential for accumulation of the protein in nucleolus, whilst co-localization results show significant overlap of BPM1 protein with aforementioned RdDM components. Yeast two hybrid assay using truncated BPM1 protein missing MATH or BTB domain revealed that the cullin3-binding BTB domain had higher affinity for RDM1 interaction while both, MATH and BTB domains proved to be equally important for DMS3 interaction. New insights into BPM1 protein and its interaction partners indicate an important, cullin independent function of MATH-BTB protein family in RdDM.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA


Projekti:
HRZZ-IP-2016-06-6229 - MATH-BTB proteini kao regulatori transkripcije i RNA posredovane metilacije DNA u biljnom razvitku (PHYTOMETHDEV) (Leljak-Levanić, Dunja, HRZZ - 2016-06) ( POIROT)

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb


Citiraj ovu publikaciju:

Jagić, Mateja; Škiljaica, Andreja; Bauer, Nataša; Leljak-Levanić, Dunja
AtBPM1 protein interacts with components of RNA- directed DNA methylation // Final Programme & Book of Abstracts: FEBS3+ From molecules to living systems / Szüts, Dávis ; Buday, László (ur.).
Veszprem, Mađarska: OOK-Press Ltd, 2018. str. 124-124 (poster, međunarodna recenzija, sažetak, znanstveni)
Jagić, M., Škiljaica, A., Bauer, N. & Leljak-Levanić, D. (2018) AtBPM1 protein interacts with components of RNA- directed DNA methylation. U: Szüts, D. & Buday, L. (ur.)Final Programme & Book of Abstracts: FEBS3+ From molecules to living systems.
@article{article, author = {Jagi\'{c}, Mateja and \v{S}kiljaica, Andreja and Bauer, Nata\v{s}a and Leljak-Levani\'{c}, Dunja}, year = {2018}, pages = {124-124}, keywords = {MATH-BTB, Arabidopsis thaliana, protein-protein interactions, RNA-directed DNA methylation}, isbn = {978-615-5270-47-5}, title = {AtBPM1 protein interacts with components of RNA- directed DNA methylation}, keyword = {MATH-BTB, Arabidopsis thaliana, protein-protein interactions, RNA-directed DNA methylation}, publisher = {OOK-Press Ltd}, publisherplace = {Si\'{o}fok, Ma\djarska} }
@article{article, author = {Jagi\'{c}, Mateja and \v{S}kiljaica, Andreja and Bauer, Nata\v{s}a and Leljak-Levani\'{c}, Dunja}, year = {2018}, pages = {124-124}, keywords = {MATH-BTB, Arabidopsis thaliana, protein-protein interactions, RNA-directed DNA methylation}, isbn = {978-615-5270-47-5}, title = {AtBPM1 protein interacts with components of RNA- directed DNA methylation}, keyword = {MATH-BTB, Arabidopsis thaliana, protein-protein interactions, RNA-directed DNA methylation}, publisher = {OOK-Press Ltd}, publisherplace = {Si\'{o}fok, Ma\djarska} }




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