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Pregled bibliografske jedinice broj: 970125

Heat stress significantly stabilizes BPM1 protein in Arabidopsis thaliana seedlings


Škiljaica, Andreja; Jagić, Mateja; Markulin, Lucija; Leljak-Levanić, Dunja; Bauer, Nataša
Heat stress significantly stabilizes BPM1 protein in Arabidopsis thaliana seedlings // Final Programme & Book of Abstracts: FEBS3+ From molecules to living systems / Szüts, Dávis ; Buday, László (ur.).
Veszprem, Mađarska: OOK-Press Ltd, 2018. str. 75-75 (predavanje, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 970125 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Heat stress significantly stabilizes BPM1 protein in Arabidopsis thaliana seedlings

Autori
Škiljaica, Andreja ; Jagić, Mateja ; Markulin, Lucija ; Leljak-Levanić, Dunja ; Bauer, Nataša

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Final Programme & Book of Abstracts: FEBS3+ From molecules to living systems / Szüts, Dávis ; Buday, László - Veszprem, Mađarska : OOK-Press Ltd, 2018, 75-75

ISBN
978-615-5270-47-5

Skup
FEBS3+ From molecules to living systems

Mjesto i datum
Siófok, Mađarska, 02-05.09.2018

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Arabidopsis, BPM1, heat stress, immunodetection

Sažetak
MATH-BTB proteins are comprised of two domains, MATH (Meprin and TRAF Homology) and BTB (Bric- A-Brac, Tramtrack, Broad Complex). While the MATH domain recognizes specific substrates, BTB domain binds Cullin 3 (CUL3) of the E3 ubiquitin ligase complex which ubiquitinates and thereby designates target proteins for proteasomal degradation. The MATH-BTB gene family is represented by six members in the Arabidopsis genome (AtBPM1-6). AtBTB proteins interact with members of at least three families of transcription factors (R2R3 MYB family, ethylene response factor/Apetala2 and class I homeobox-leucine zipper transcription factors) which play important roles during plant flowering, seed development and abiotic stress response. Here, transgenic A. thaliana plants with overexpression of GFP-tagged BPM1 were successfully regenerated and analyzed, exhibiting early flowering phenotype and resistance to ABA. Despite high overexpression rate, recombinant BPM1 protein was unstable under control conditions. The stability of BPM1 protein was analyzed after treatment of transgenic seedlings in conditions of salt and osmotic stress (150 mM NaCl or 300 mM mannitol), darkness, elevated temperatures, and upon heat stress. Seedlings were collected for whole soluble protein extraction and anti-GFP monoclonal antibody was used to blot transgenic GFP-tagged proteins. Darkness and salt stress induced degradation of GFP-BPM1, as opposed to heat treatment which caused significant accumulation of GFP-BPM1. Improved stabilization of transgenic GFP-BPM1 in conditions of elevated temperature could indicate the physiological role of AtBPM1 in response to heat stress.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA


Projekti:
HRZZ-IP-2016-06-6229 - MATH-BTB proteini kao regulatori transkripcije i RNA posredovane metilacije DNA u biljnom razvitku (PHYTOMETHDEV) (Leljak-Levanić, Dunja, HRZZ - 2016-06) ( POIROT)

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb


Citiraj ovu publikaciju:

Škiljaica, Andreja; Jagić, Mateja; Markulin, Lucija; Leljak-Levanić, Dunja; Bauer, Nataša
Heat stress significantly stabilizes BPM1 protein in Arabidopsis thaliana seedlings // Final Programme & Book of Abstracts: FEBS3+ From molecules to living systems / Szüts, Dávis ; Buday, László (ur.).
Veszprem, Mađarska: OOK-Press Ltd, 2018. str. 75-75 (predavanje, međunarodna recenzija, sažetak, znanstveni)
Škiljaica, A., Jagić, M., Markulin, L., Leljak-Levanić, D. & Bauer, N. (2018) Heat stress significantly stabilizes BPM1 protein in Arabidopsis thaliana seedlings. U: Szüts, D. & Buday, L. (ur.)Final Programme & Book of Abstracts: FEBS3+ From molecules to living systems.
@article{article, author = {\v{S}kiljaica, Andreja and Jagi\'{c}, Mateja and Markulin, Lucija and Leljak-Levani\'{c}, Dunja and Bauer, Nata\v{s}a}, year = {2018}, pages = {75-75}, keywords = {Arabidopsis, BPM1, heat stress, immunodetection}, isbn = {978-615-5270-47-5}, title = {Heat stress significantly stabilizes BPM1 protein in Arabidopsis thaliana seedlings}, keyword = {Arabidopsis, BPM1, heat stress, immunodetection}, publisher = {OOK-Press Ltd}, publisherplace = {Si\'{o}fok, Ma\djarska} }
@article{article, author = {\v{S}kiljaica, Andreja and Jagi\'{c}, Mateja and Markulin, Lucija and Leljak-Levani\'{c}, Dunja and Bauer, Nata\v{s}a}, year = {2018}, pages = {75-75}, keywords = {Arabidopsis, BPM1, heat stress, immunodetection}, isbn = {978-615-5270-47-5}, title = {Heat stress significantly stabilizes BPM1 protein in Arabidopsis thaliana seedlings}, keyword = {Arabidopsis, BPM1, heat stress, immunodetection}, publisher = {OOK-Press Ltd}, publisherplace = {Si\'{o}fok, Ma\djarska} }




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