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Wheat MATH-BTB proteins and their role in early embryogenesis

The isolation of egg cells, zygotes and two-celled proembryos from embryo sacs of wheat (Triticum aestivum L.) resulted in trustworthy cDNA libraries, based on which the presence of TaMAB2 protein was detected in the zygote and two-celled embryo. TaMAB2 protein belongs to a family of proteins with two domains: MATH (Meprin and TRAF homology) and BTB (Brick-a-brack/tramtrack/broad complex). MATH-BTB family is widely represented among eukaryotes. NCBI and EnsemblPlants databases were searched using the amino acid sequence of TaMAB2 protein as query, revealing 46 novel wheat MATHBTB proteins. Using Clustal X and Seaview, a phylogenetic analysis of MATH-BTB genes of wheat, rice (Oryza sativa), maize (Zea mays) and Arabidopsis thaliana was conducted, with TaMAB2 protein clustering into the E3 subclade. Multiple sequence alignments of selected proteins in MUSCLE and Boxshade indicated a significant sequence similarity between all proteins of the E3 subclade, which suggesed their possible functional similarity. A yeast two-hybrid assay was conducted in order to analyze potential interaction partners of TaMAB2 protein. This revealed Katanin protein as a plausible physiological substrate of TaMAB2. Phenotype analysis of TaMAB2 overexpression line of arabidopsis indicated inhibited growth and development of adult arabidopsis plants as well as decreased frequency of somatic embryogenesis in in culture.

MATH-BTB phylogeny, protein - protein interactions, TaMAB2, Katanin, somatic embryogenesis

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