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Pregled bibliografske jedinice broj: 969953

Immunohistochemical analysis of vocal cord polyps applying markers of squamous cell carcinogenesis


Braut, Tamara; Krstulja, Mira; Marijić, Blažen; Maržić, Diana; Kujundžić, Milodar; Brumini, Gordana; Vučinić, Damir; Oštarijaš, Eduard
Immunohistochemical analysis of vocal cord polyps applying markers of squamous cell carcinogenesis // Pathology - Research and Practice, 215 (2019), 1; 144-150 doi:10.1016/j.prp.2018.11.001 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 969953 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Immunohistochemical analysis of vocal cord polyps applying markers of squamous cell carcinogenesis

Autori
Braut, Tamara ; Krstulja, Mira ; Marijić, Blažen ; Maržić, Diana ; Kujundžić, Milodar ; Brumini, Gordana ; Vučinić, Damir ; Oštarijaš, Eduard

Izvornik
Pathology - Research and Practice (0344-0338) 215 (2019), 1; 144-150

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Carcinogenesis ; Hyperplasia ; Immunohistochemistry ; Laryngeal polyps ; Molecular markers

Sažetak
Objective The aim of the study was to perform a pathohistological and immunohistochemical analysis of squamous cell (SC) carcinogenesis markers on epithelial linings of vocal cord polyps. The vocal box, being a heavily burdened organ with intensive cell renewal and regenerative processes, is therefore a favourable environment for constant epithelial growth and hyperplasia. In our ongoing projects on laryngeal carcinogenesis and research on laryngeal tissue, we encountered atypia on diagnosed nodules and polyps that are usually considered as benign formations, resulting from the above-mentioned cell renewal and regeneration, which lead to further investigation. The purpose was to see if changes in molecular markers of SC carcinogenesis follow, or, may appear in immunohistochemical (IHC) analysis, before histological atypia in standard haematoxylin- eosin (HE) staining, and contribute in early diagnosis of potentially suspect polyps. Methods After classical pathohistological (PH) analysis on HE slides, IHC analysis of EGFR, cyclin D1, p53, Ki-67, and IMP3 was performed on tissue microarrays of laryngeal tissue (50 samples), ranging from normal to hyperplastic lesions with no atypia (34 samples), low-grade atypia (11 samples), and high-grade atypia (5 samples). Results This study established an increase and correlation of EGFR, cyclin D1, p53, Ki-67 and IMP3 IHC expressions with pathohistological findings of dysplasia in glottic polypoid lesions. Low and high-grade dysplasia had statistically higher percentages of EGFR- positive cells than normal epithelium and simple hyperplasia (SH) (low vs. normal/SH P = 0.007 ; high vs. normal/SH P = 0.001). High-grade dysplasia had statistically more positive cells than low-grade dysplasia (P = 0.004), and low- grade dysplasia had statistically more positive cells than specimens without atypia (P = 0.007). The percentage of positive cells was statistically higher for cyclin D1, p53 and Ki- 67 in high- grade dysplasia versus low-grade dysplasia (cyclin D1 P = 0.011, p53 P = 0.002 ; Ki-67 P = 0.026 ; respectively) and versus normal epithelium and SH (cyclin D1 P = 0.003 ; p53 P = 0.001 ; Ki-67 P = 0.002 ; respectively). An increase of IMP3- positive cells with an increase of atypical changes in the laryngeal epithelium, from superficial towards basal layers was noticed, contrary to the usually seen positivity pattern of SC carcinogenesis markers from basal to superficial layers. A statistically significant difference of IMP3 IHC staining between the pathohistological groups (P = 0.003) was recorded. Conclusion Only polyps that present with simple hyperplasia as the greatest mucosal change can be considered as benign formations. Pathohistologically detected atypia in polypoid changes of vocal cords, confirmed by molecular atypia with an increase of SC carcinogenesis markers, suggest their inclusion in studies of laryngeal carcinogenesis. Our results suggest that in problematic cases IHC analysis could be of interest in detection of biological aggressiveness in polypoid laryngeal tissue and beneficiary for polyp patients’ follow-up. Further research of laryngeal carcinogenesis markers and their meaning in fibrovascular polyps is of interest.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA


Ustanove:
Medicinski fakultet, Rijeka,
Klinički bolnički centar Rijeka,
Fakultet zdravstvenih studija u Rijeci

Citiraj ovu publikaciju

Braut, Tamara; Krstulja, Mira; Marijić, Blažen; Maržić, Diana; Kujundžić, Milodar; Brumini, Gordana; Vučinić, Damir; Oštarijaš, Eduard
Immunohistochemical analysis of vocal cord polyps applying markers of squamous cell carcinogenesis // Pathology - Research and Practice, 215 (2019), 1; 144-150 doi:10.1016/j.prp.2018.11.001 (međunarodna recenzija, članak, znanstveni)
Braut, T., Krstulja, M., Marijić, B., Maržić, D., Kujundžić, M., Brumini, G., Vučinić, D. & Oštarijaš, E. (2019) Immunohistochemical analysis of vocal cord polyps applying markers of squamous cell carcinogenesis. Pathology - Research and Practice, 215 (1), 144-150 doi:10.1016/j.prp.2018.11.001.
@article{article, year = {2019}, pages = {144-150}, DOI = {10.1016/j.prp.2018.11.001}, keywords = {Carcinogenesis, Hyperplasia, Immunohistochemistry, Laryngeal polyps, Molecular markers}, journal = {Pathology - Research and Practice}, doi = {10.1016/j.prp.2018.11.001}, volume = {215}, number = {1}, issn = {0344-0338}, title = {Immunohistochemical analysis of vocal cord polyps applying markers of squamous cell carcinogenesis}, keyword = {Carcinogenesis, Hyperplasia, Immunohistochemistry, Laryngeal polyps, Molecular markers} }
@article{article, year = {2019}, pages = {144-150}, DOI = {10.1016/j.prp.2018.11.001}, keywords = {Carcinogenesis, Hyperplasia, Immunohistochemistry, Laryngeal polyps, Molecular markers}, journal = {Pathology - Research and Practice}, doi = {10.1016/j.prp.2018.11.001}, volume = {215}, number = {1}, issn = {0344-0338}, title = {Immunohistochemical analysis of vocal cord polyps applying markers of squamous cell carcinogenesis}, keyword = {Carcinogenesis, Hyperplasia, Immunohistochemistry, Laryngeal polyps, Molecular markers} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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