engleski

A laboratory scale transglucosylation by using whole cells as biocatalysts

Cultivation of L. mesenteroides, permeabilization of cultivated cells, and transglucosylation reaction catalyzed by the permeablized L. mesenteroides cells will be investigated. Cultivation parameters are to be optimized in order to achieve suitable growth of L. mesenteroides on sucrose without fructose conversion to mannitol and also production of other end-products of central metabolism (e.g. acetate and ethanol). Different physical and chemical methods will be screened in order to improve L. mesenteroides cell membrane permeability, like ultrasound treatment and addition of surfactants, and most efficient and sustainable method will be used for preparation of biocatalyst for the laboratory scale transglucosylation. In the whole-cells catalyzed reaction, sucrose and glycerol will be used as glucosyl donor and glucosyl acceptor, respectively, in order to synthesize 2-O-(α-D-glucopyranosyl)-sn-glycerol. Optimal ratio of the two reactants for an highly efficient transglucosylation reaction will be optimized. In a collaboration with a scientific partner we aim to optimize a detailed set of procedures for (i) the cultivation of L. mesenteroides, (ii) the permeabilization of cultivated cells, and (iii) the transglucosylation reaction catalyzed by the permeabilized L. mesenteroides cells. Different concentrations of the biocatalyst, donor and acceptor molecules, as well as a range of bioprocess parameters, primary pH and temperature, will be combined in order to increase transglucosylation efficiency at the laboratory scale.

whole-cells, transglucosylation, laboratory scale

nije evidentirano