Naslov
Power of uncharacterized Lactobacillus amylovorus DSM 20531T amylase(s)

Autori
Polenus, Antonela ; Slavica, Anita

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
European Summit of Industrial Biotechnology 2017 (esib 2017) / Berg, Gabriele et al. - Graz : Acib, 2017, 174-174

Skup
European Summit of Industrial Biotechnology 2017 (esib 2017)

Mjesto i datum
Graz, Austrija, 14.11.2017. - 16.11.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Lactobacillus amylovorus, amylase, semi-solid substrates

Sažetak
Wild-type L. amylovorus DSM 20531T produces apparently amylolytic enzymes that are sensitive toward extracellular proteolysis. Three forms ot active enzymes were separated by ion-exchange chromatography and also by affinity chromatography. De novo sequencing of peptides, obtained from the separated active amylolytic enzymes, from tandem mass spectrometry data has been attracting our attention. The complete genome of L. amylovorus DSM 20531T and the nucleotide sequence of the amylase(s) and the protease genes have not yet been determined. It is of utmost importance to apply know-how of a research group who will help us with: sequencing genome of the strain, identification of the enzymes-coding genes, finding convenient vector and overexpression of the enzymes-coding genes in a suitable host. We offer a co-development of the genetically engineered L. amylovorus strain capable of producing the inermediates and the platform chemical from raw materials under moderate conditions.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA