Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 969216

Leuconostoc mesenteroides whole-cell catalysed glucosylation


Vučenović, Ivan; Brnčić, Mladen; Slavica, Anita
Leuconostoc mesenteroides whole-cell catalysed glucosylation // Journal of Biotechnology / Dundar, Munis (ur.).
Athens, Greece: Elsevier Ltd., 2018. str. S44-S44 (poster, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 969216 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Leuconostoc mesenteroides whole-cell catalysed glucosylation

Autori
Vučenović, Ivan ; Brnčić, Mladen ; Slavica, Anita

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Journal of Biotechnology / Dundar, Munis - : Elsevier Ltd., 2018, S44-S44

Skup
European Biotechnology Congress 2018, Athens, Greece 26-28 April 2018

Mjesto i datum
Athens, Greece, 26.-28.04.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cell permeabilization, Leuconostoc mesenteroides, sucrose, glucosylation

Sažetak
Leuconostoc mesenteroides belongs to the group of industrially relevant biocatalysts and its modern implementation in a manufacturing of whole range of mass products is far beyond acidification and flavouring of certain feedstock. Genetic potential of this mesophylic heterofermentative lactic acid bacterium comprises functional gene(s) for sucrose phosphporylase (EC 2.4.1.7), very well characterized enzyme used for glucosylation reactions in the industrial scale. In this work we investigated employment of permeabilized cells of L. mesenteroides in order to perform targeted glucosylation reaction in which sucrose served as an glucosyl donor while glycerol was a glucosyl acceptor. The bacterium was cultivated in the semi-defined medium during batch experiments that were carried out at 29±1C and nonmaintained pH value, and harvested cells where permeabilized by (i) ultrasound treatment (37 kHz, 90 W, 29±1℃ continuously over 4 hours and periodically two times over 30 minutes with 30 minutes pause inbetween the each treatment) and (ii) non- ionic detergent Tween-80 (0.4% and 0.6%). Possible formation of α-D-glucosylglycerol was indicated by a new peak that was eluted from ion-exchange column between sucrose and fructose. To estimate efficiency of performed transglucosylation reaction, separation, concentration and additional characterization of potential product is needed.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb

Profili:

Avatar Url Anita Slavica (autor)

Avatar Url Mladen Brnčić (autor)


Citiraj ovu publikaciju:

Vučenović, Ivan; Brnčić, Mladen; Slavica, Anita
Leuconostoc mesenteroides whole-cell catalysed glucosylation // Journal of Biotechnology / Dundar, Munis (ur.).
Athens, Greece: Elsevier Ltd., 2018. str. S44-S44 (poster, međunarodna recenzija, sažetak, znanstveni)
Vučenović, I., Brnčić, M. & Slavica, A. (2018) Leuconostoc mesenteroides whole-cell catalysed glucosylation. U: Dundar, M. (ur.)Journal of Biotechnology.
@article{article, editor = {Dundar, M.}, year = {2018}, pages = {S44-S44}, keywords = {cell permeabilization, Leuconostoc mesenteroides, sucrose, glucosylation}, title = {Leuconostoc mesenteroides whole-cell catalysed glucosylation}, keyword = {cell permeabilization, Leuconostoc mesenteroides, sucrose, glucosylation}, publisher = {Elsevier Ltd.}, publisherplace = {Athens, Greece} }
@article{article, editor = {Dundar, M.}, year = {2018}, pages = {S44-S44}, keywords = {cell permeabilization, Leuconostoc mesenteroides, sucrose, glucosylation}, title = {Leuconostoc mesenteroides whole-cell catalysed glucosylation}, keyword = {cell permeabilization, Leuconostoc mesenteroides, sucrose, glucosylation}, publisher = {Elsevier Ltd.}, publisherplace = {Athens, Greece} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
    • Conference Proceedings Citation Index - Science (CPCI-S)
    • Conference Proceedings Citation Index - Social Sciences & Humanities (CPCI-SSH)
  • Scopus
  • MEDLINE





Contrast
Increase Font
Decrease Font
Dyslexic Font