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Pregled bibliografske jedinice broj: 969078

Concept of sample-specific correction of immunoassay results for precise and accurate IgG quantification in horse plasma


Halassy, Beata; Kurtović, Tihana; Lang Balija, Maja; Brgles, Marija; Tunjić, Monika; Sviben, Dora
Concept of sample-specific correction of immunoassay results for precise and accurate IgG quantification in horse plasma // Journal of pharmaceutical and biomedical analysis, 164 (2019), 276-282 doi:10.1016/j.jpba.2018.10.020 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 969078 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Concept of sample-specific correction of immunoassay results for precise and accurate IgG quantification in horse plasma

Autori
Halassy, Beata ; Kurtović, Tihana ; Lang Balija, Maja ; Brgles, Marija ; Tunjić, Monika ; Sviben, Dora

Izvornik
Journal of pharmaceutical and biomedical analysis (0731-7085) 164 (2019); 276-282

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Antitoxin downstream processing ; Antivenom ; IgG quantification ; Hyperimmune horse plasma ; ELISA ; IgG subclass distribution

Sažetak
The hyperimmune horse plasma (HHP), prepared through active immunisation of horses with an antigen of interest, is the most common starting material for antitoxin (animal antibody-based therapeutics) production. Precise IgG quantification in plasma is a prerequisite for accurate estimation of the purification process efficiency. Although immunoglobulins from HHP have been purified for over a century, there is still no in vitro method for precise and accurate determination of IgG content in HHP. For this reason, the purification process efficiency has been assessed by antibody activity measurements, mostly performed in vivo. Here we describe the development of a precise and accurate in vitro immunoassay for IgG quantification in HHP. We showed and highlighted that any difference in composition of IgG population between the standard and the sample, with respect to both IgG subclass distribution and antigen-specific IgG content, leads to inaccurate IgG quantification. We demonstrated that caprylic acid precipitation as the method for IgG isolation from horse plasma renders the composition of IgG population unchanged. This very efficient, fast, simple and inexpensive method was used to prepare internal, sample- specific reference IgG for each plasma sample, which was tested simultaneously to a respective plasma sample. Deviation of IgG quantity determined by ELISA for each sample-specific reference from its nominal value was used for correction of the results of respective plasma sample, which led to accurate and precise IgG quantification as shown by method validation. The here presented novel concept of sample- specific correction of immunoassay results could be widely applicable and easily introduced in different immunoassays for more accurate and precise plasma IgG quantification.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA


Projekti:
HRZZ-IP-2014-09-4915 - Razvoj održivog procesa prerade antitoksina (ANTI TOX NEW) (Halassy, Beata, HRZZ - 2014-09) ( POIROT)

Ustanove:
Sveučilište u Zagrebu

doi

Citiraj ovu publikaciju

Halassy, Beata; Kurtović, Tihana; Lang Balija, Maja; Brgles, Marija; Tunjić, Monika; Sviben, Dora
Concept of sample-specific correction of immunoassay results for precise and accurate IgG quantification in horse plasma // Journal of pharmaceutical and biomedical analysis, 164 (2019), 276-282 doi:10.1016/j.jpba.2018.10.020 (međunarodna recenzija, članak, znanstveni)
Halassy, B., Kurtović, T., Lang Balija, M., Brgles, M., Tunjić, M. & Sviben, D. (2019) Concept of sample-specific correction of immunoassay results for precise and accurate IgG quantification in horse plasma. Journal of pharmaceutical and biomedical analysis, 164, 276-282 doi:10.1016/j.jpba.2018.10.020.
@article{article, year = {2019}, pages = {276-282}, DOI = {10.1016/j.jpba.2018.10.020}, keywords = {Antitoxin downstream processing, Antivenom, IgG quantification, Hyperimmune horse plasma, ELISA, IgG subclass distribution}, journal = {Journal of pharmaceutical and biomedical analysis}, doi = {10.1016/j.jpba.2018.10.020}, volume = {164}, issn = {0731-7085}, title = {Concept of sample-specific correction of immunoassay results for precise and accurate IgG quantification in horse plasma}, keyword = {Antitoxin downstream processing, Antivenom, IgG quantification, Hyperimmune horse plasma, ELISA, IgG subclass distribution} }
@article{article, year = {2019}, pages = {276-282}, DOI = {10.1016/j.jpba.2018.10.020}, keywords = {Antitoxin downstream processing, Antivenom, IgG quantification, Hyperimmune horse plasma, ELISA, IgG subclass distribution}, journal = {Journal of pharmaceutical and biomedical analysis}, doi = {10.1016/j.jpba.2018.10.020}, volume = {164}, issn = {0731-7085}, title = {Concept of sample-specific correction of immunoassay results for precise and accurate IgG quantification in horse plasma}, keyword = {Antitoxin downstream processing, Antivenom, IgG quantification, Hyperimmune horse plasma, ELISA, IgG subclass distribution} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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