Napredna pretraga

Pregled bibliografske jedinice broj: 968919

Evolution of MHC class II SLA-DRB1 locus in the Croatian wild boar (Sus scrofa) implies duplication and weak signals of positive selection


Arbanasić, Haidi; Konjević, Dean; Vranković, Lana; Bujanić, Miljenko; Stipoljev, Sunčica; Balažin, Maja; Šprem, Nikica; Škorić, Dijana; Galov, Ana
Evolution of MHC class II SLA-DRB1 locus in the Croatian wild boar (Sus scrofa) implies duplication and weak signals of positive selection // Animal genetics, 50 (2019), 1; 33-41 doi:10.1111/age.12734 (međunarodna recenzija, članak, znanstveni)


Naslov
Evolution of MHC class II SLA-DRB1 locus in the Croatian wild boar (Sus scrofa) implies duplication and weak signals of positive selection

Autori
Arbanasić, Haidi ; Konjević, Dean ; Vranković, Lana ; Bujanić, Miljenko ; Stipoljev, Sunčica ; Balažin, Maja ; Šprem, Nikica ; Škorić, Dijana ; Galov, Ana

Izvornik
Animal genetics (0268-9146) 50 (2019), 1; 33-41

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DRB, locus duplication, SLA, three-allelic individuals, two-locus haplotype

Sažetak
The wild boar is an ancestor of the domestic pig and an important game species with the widest geographical range of all ungulates. Although a large amount of data are available on major histocompatibility complex (MHC) variability in domestic pigs, only a few studies have been performed on wild boars. Due to their crucial role in appropriate immune responses and extreme polymorphism, MHC genes represent some of the best candidates for studying the processes of adaptive evolution. Here, we present the results on the variability and evolution of the entire MHC class II SLA- DRB1 locus exon 2 in 133 wild boars from Croatia. Using direct sequencing and cloning methods, we identified 20 SLA-DRB1 alleles, including eight new variants, with notable divergence. In some individuals, we documented functional locus duplication, and SLA- DRB1*04:10 was identified as the allele involved in the duplication. The expression of a duplicated locus was confirmed by cloning and sequencing cDNA-derived amplicons. Based on individual genotypes, we were able to assume that alleles SLA-DRB1*04:10 and SLA- DRB1*06:07 are linked as an allelic combination that co-evolves as a two-locus haplotype. Our investigation of evolutionary processes at the SLA-DRB1 locus confirmed the role of intralocus recombination in generating allelic variability, whereas tests of positive selection based on the dN/dS (nonsynonymous/synonymous substitution rate ratio) test revealed atypically weak and ambiguous signals.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Veterinarska medicina



POVEZANOST RADA


Projekt / tema
HRZZ-UIP-2013-11-3421 - Molekularna epidemiologija nekih invazijskih oboljenja divljih životinja (Dean Konjević, )

Ustanove
Veterinarski fakultet, Zagreb,
Prirodoslovno-matematički fakultet, Zagreb,
Agronomski fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati