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Development of Near Infrared Spectroscopy Models for the Quantitative Prediction of Olive Leaves Bioactive Compounds Content

The objective of this work was to evaluate the ability of Artificial Neural Networks (ANN) in Near Infrared (NIR) spectra calibration models to predict the total polyphenols content, antioxidant activity and extraction yield of the olive leaves aqueous extracts, prepared with three extraction procedures (conventional extraction, microwave-assisted extraction, and microwave–ultrasound-assisted extraction). Partial Least Square (PLS) models were developed formed from Principle Component Analyses (PCA) scores of NIR spectra of olive leaves aqueous extracts in terms of total polyphenols concentration, antioxidant activity and extraction yield, for each of extraction procedure. PLS models were used to view which PCA scores are the best suited as input for ANN based on three output variables. ANN showed very good correlation of NIRs and all tested variables especially in the case of Total Polyphenolic Content (TPC). Therefore, ANN can be used for the prediction of total polyphenol concentrations, antioxidant activity and extraction yield of plant extracts based on the NIR spectra.

NIR spectra, artificial neural networks, olive leaves extracts, conventional extraction, microwave-assisted extraction, microwave–ultrasound-assisted extraction

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