engleski

Lung polar lipid fatty acids composition in the mice after feeding different lipid supplemented diets

This study investigated the potential of dietary fats to modulate the fatty acid composition of mice lung phospholipids. Changing the type and amount of lipids in the diet alters the FA composition of the lung surfactant, which may change the physical and physiological properties of surfactant and the alveolar surface tension. Fatty acid composition in lung polar lipids was determined in 2-3 mo old male Balb/cN mice after three weeks of feeding lipid-supplemented diets containing olive oil (5% w/w, FOO group) and corn oil (5% w/w, FCO group). Total lipids were extracted according to Folch et al., and polar lipids were separated and purified by solid-phase extraction and analysed in form of the fatty acid methyl esters by gas chromatographic analysis. Differences among groups were tested by nonparametric Kruskal-Wallis and Mann-Whitney U-test. Similar to total lipids, phospholipids were fatty acids affected by the type of dietary oils in the examined tissue. Palmitic and stearic acids were major saturated fatty acids in polar lipids in the lung tissue. Feeding lipid-supplemented diet reduced C18:2/C20:4 ratio in the FOO and increased in FCO groups. The oleic acid content significantly increased in the FOO diet than in the control and FCO group and was the major monounsaturated in the lung polar lipid tissue. Docosahexaenoic acid (DHA) was the major polyunsaturated in the lung tissue sample, higher content of DHA was in FOO diet than in the control and FCO group.

Lung ; Fatty acids ; Phospholipids ; Corn oil diet ; Olive oil die

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