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Assessment of acetylcholinesterase activity, oxidative stress responses, and primary DNA damage in blood and brain tissue of chlorpyrifos-exposed rats


Lucić Vrdoljak, Ana; Žunec, Suzana; Fuchs, Radovan; Kopjar Nevenka
Assessment of acetylcholinesterase activity, oxidative stress responses, and primary DNA damage in blood and brain tissue of chlorpyrifos-exposed rats // Proceedings of 2nd International Conference on Pollution Control and Sustainable Environment / Liao, Shao Wei (ur.).
London: conferenceseries.com, 2017. str. 50-50 doi:10.4172/2375-4397-C1-009 (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
Assessment of acetylcholinesterase activity, oxidative stress responses, and primary DNA damage in blood and brain tissue of chlorpyrifos-exposed rats

Autori
Lucić Vrdoljak, Ana ; Žunec, Suzana ; Fuchs, Radovan ; Kopjar Nevenka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of 2nd International Conference on Pollution Control and Sustainable Environment / Liao, Shao Wei - London : Conferenceseries.com, 2017, 50-50

Skup
2nd International Conference on Pollution Control and Sustainable Environment

Mjesto i datum
London, Ujedinjeno Kraljevstvo, 05.-06. 10.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Organophosphate compounds ; Insecticide ; DNA instability ; In vivo

Sažetak
Organophosphate compounds are among the most frequently used pesticides worldwide. In this study, we evaluated the in vivo effects of the insecticide chlorpyrifos, which was orally administered to male Wistar rats at 0.160 mg/kg, 0.015 mg/kg, and 0.010 mg/kg for 28 days. Following treatment, the level of lipid peroxidation and acetylcholinesterase (AChE) activity was estimated in plasma and brain. To establish the potential DNA damaging effects of the exposure, we applied an alkaline comet assay on the white blood cells and brain tissue of the exposed and control animals. We found that 28-day exposure resulted in dose-dependent changes in AChE activity, which was significantly more depressed in the brain. Lipid peroxidation, presented as TBARS concentration, was elevated both in plasma and in the brain. All of the tested doses of chlorpyrifos were slightly genotoxic, both to the white blood cells and brain tissue. Taken together, our findings confirmed the AChE-inhibiting potency of chlorpyrifos and indicated that its toxicity was mediated through free radicals, which contributed to DNA instability. Considering that our study focused on very low doses of chlorpyrifos within toxicology reference values our results call for further research using other sensitive biomarkers of effect, along with different exposure scenarios.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2013-11-8366 - Organska zagađivala u okolišu - markeri i biomarkeri toksičnosti (Davor Želježić, )

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb

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