Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 964933

Extracellular Hsp70 induces inflammation and modulates LPS/LTA-stimulated inflammatory response in THP-1 cells


Hulina, Andrea; Grdić Rajković, Marija; Jakšić Despot, Daniela; Jelić, Dubravko; Dojder, Ana; Čepelak, Ivana; Rumora, Lada
Extracellular Hsp70 induces inflammation and modulates LPS/LTA-stimulated inflammatory response in THP-1 cells // Cell stress & chaperones, 23 (2018), 3; 373-384 doi:10.1007/s12192-017-0847-0 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 964933 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Extracellular Hsp70 induces inflammation and modulates LPS/LTA-stimulated inflammatory response in THP-1 cells

Autori
Hulina, Andrea ; Grdić Rajković, Marija ; Jakšić Despot, Daniela ; Jelić, Dubravko ; Dojder, Ana ; Čepelak, Ivana ; Rumora, Lada

Izvornik
Cell stress & chaperones (1355-8145) 23 (2018), 3; 373-384

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
THP-1 cells, extracellularHsp70, LPS, LTA, COPD

Sažetak
Extracellular Hsp70 (eHsp70) can act as damageassociated molecular pattern (DAMP) via Toll-like receptors TLR2 and TLR4, and stimulate immune and inflammatory responses leading to sterile inflammation and propagation of already existing inflammation. It was found elevated in the blood of patients with chronic obstructive pulmonary disease (COPD), who might suffer occasional bacterial colonizations and infections. We used a monocytic THP-1 cell line as a cellular model of systemic compartment of COPD to assess inflammatory effects of eHsp70 when present alone or together with bacterial products lypopolysaccharide (LPS) and lypoteichoic acid (LTA). THP-1 cells were differentiated into macrophage-like cells and treated with various concentrations of recombinant human Hsp70 protein (rhHsp70), LPS (TLR4 agonist), LTA (TLR2 agonist), and their combinations for 4, 12, 24, and 48 h. Concentrations of IL-1α, IL-6, IL-8, and TNF-α were determined by ELISA. Cell viability was assessed by MTS assay, and mode of cell death by luminometric measurements of caspases-3/7, -8, and -9 activities. rhHsp70 showed cell protecting effect by suppressing caspases-3/7 activation, while LPS provoked cytotoxicity through caspases-8 and -3/7 pathway. Regarding inflammatory processes, rhHsp70 alone induced secretion of IL-1α and IL-8, but had modulatory effects on release of all four cytokines when applied together with LPS or LTA. Combined effect with LPS was mainly synergistic, and with LTA mainly antagonistic, although it was cytokine- and timedependent. Our results confirmed pro inflammatory function of extracellular Hsp70, and suggest its possible implication in COPD exacerbations caused by bacterial infection through desensitization or inappropriate activation of TLR2 and TLR4 receptors.

Izvorni jezik
Engleski

Znanstvena područja
Interdisciplinarne prirodne znanosti, Temeljne medicinske znanosti, Kliničke medicinske znanosti, Farmacija

Napomena
Rad/časopis indeksiran je i u: EBSCO ; Google Scholar ; JCR/Science EditionGale ; Gale Academic OneFile ; JSTOR ; OCLC WorldCat Discovery ServicePathway Studi ; ProQuest ; Reaxys.



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2014-09-1247 - Uloga stresnog proteina Hsp70 u imunosno-upalnom odgovoru kod kronične opstrukcijske plućne bolesti (Hsp70COPD) (, HRZZ - 2014-09 )

Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb,
Fidelta d.o.o.

Citiraj ovu publikaciju

Hulina, Andrea; Grdić Rajković, Marija; Jakšić Despot, Daniela; Jelić, Dubravko; Dojder, Ana; Čepelak, Ivana; Rumora, Lada
Extracellular Hsp70 induces inflammation and modulates LPS/LTA-stimulated inflammatory response in THP-1 cells // Cell stress & chaperones, 23 (2018), 3; 373-384 doi:10.1007/s12192-017-0847-0 (međunarodna recenzija, članak, znanstveni)
Hulina, A., Grdić Rajković, M., Jakšić Despot, D., Jelić, D., Dojder, A., Čepelak, I. & Rumora, L. (2018) Extracellular Hsp70 induces inflammation and modulates LPS/LTA-stimulated inflammatory response in THP-1 cells. Cell stress & chaperones, 23 (3), 373-384 doi:10.1007/s12192-017-0847-0.
@article{article, year = {2018}, pages = {373-384}, DOI = {10.1007/s12192-017-0847-0}, keywords = {THP-1 cells, extracellularHsp70, LPS, LTA, COPD}, journal = {Cell stress and chaperones}, doi = {10.1007/s12192-017-0847-0}, volume = {23}, number = {3}, issn = {1355-8145}, title = {Extracellular Hsp70 induces inflammation and modulates LPS/LTA-stimulated inflammatory response in THP-1 cells}, keyword = {THP-1 cells, extracellularHsp70, LPS, LTA, COPD} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • Algology Mycology and Protozoology Abstracts (Microbiology C)
  • BIOSIS Previews (Biological Abstracts)
  • Calcium and Calcified Tissue Abstracts
  • CA Search (Chemical Abstracts)
  • EMBASE (Excerpta Medica)
  • Industrial and Applied Microbiology Abstracts (Microbiology A)
  • Nucleic Acids Abstracts
  • Virology and AIDS Abstracts
  • Zoological Record


Citati





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font