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The autophagy plays an important role in intracellular life of Francisella within Dictyostelium cells.


Ožanič, Mateja; Nora, Valerija; Marečić, Valentina; Mihelčić; Šantić, Marina
The autophagy plays an important role in intracellular life of Francisella within Dictyostelium cells. // 9th International Conference on Tularemia / Elkins, Karen (ur.).
Montreal: TULISOC, 2018. str. 109-109 (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
The autophagy plays an important role in intracellular life of Francisella within Dictyostelium cells.

Autori
Ožanič, Mateja ; Nora, Valerija ; Marečić, Valentina ; Mihelčić ; Šantić, Marina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
9th International Conference on Tularemia / Elkins, Karen - Montreal : TULISOC, 2018, 109-109

Skup
„9th International conference on Tularemia“

Mjesto i datum
Montreal, Kanada, 15-19.10.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Dictyostelium, autophagy, intracellular lifestyle

Sažetak
Autophagy or autophagocytosis is an evolutionally preserved catabolic process that involves the degradation of cytoplasmic components. During infection, various microorganisms regulate the autophagy process differently. Studies have shown that F. novicida in some cells stimulate autophagy, and in others suppress the autophagy process in favor of intracellular replication. Most of the studies were performed in mammalian cells, and very little is known about the autophagy process in the amoeba cell after infection with Francisella. It has been shown that in Dictyostelium, F. noatunensis subsp. noatunensis interacts with the autophagic machinery. There are no data about the role of autophagy of survival and replication of F. novicida and F. tularensis subsp. holarctica in Dictyostelium discoideum. In this study, we monitored the autophagy process in this social amoeba using different inducers and inhibitors of autophagy over infection with F. novicida and F. tularensis subsp. holarctica. The results have shown that treatment of cells with autophagy inhibitors, chloroquine and wortmannin has a negative effect on survival, replication and intracellular trafficking of both strains of Francisella. In contrast, induction of autophagy in amoebae cells results of high number of intracellular bacteria, successful replication in Francisella containing vacuole. We can conclude that the formation of an autophagic vacuole support the intracellular lifestyle of Francisella within Dictyostelium discoideum.

Izvorni jezik
Engleski



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2016-06-9003 - Uloga unutarstaničnog života Francisella tularensis u patogenezi eksperimentalne tularemije (Marina Šantić, )
UNIRI 811.10.1111

Citiraj ovu publikaciju

Ožanič, Mateja; Nora, Valerija; Marečić, Valentina; Mihelčić; Šantić, Marina
The autophagy plays an important role in intracellular life of Francisella within Dictyostelium cells. // 9th International Conference on Tularemia / Elkins, Karen (ur.).
Montreal: TULISOC, 2018. str. 109-109 (poster, međunarodna recenzija, sažetak, znanstveni)
Ožanič, M., Nora, V., Marečić, V., Mihelčić & Šantić, M. (2018) The autophagy plays an important role in intracellular life of Francisella within Dictyostelium cells.. U: Elkins, K. (ur.)9th International Conference on Tularemia.
@article{article, editor = {Elkins, K.}, year = {2018}, pages = {109-109}, keywords = {Dictyostelium, autophagy, intracellular lifestyle}, title = {The autophagy plays an important role in intracellular life of Francisella within Dictyostelium cells.}, keyword = {Dictyostelium, autophagy, intracellular lifestyle}, publisher = {TULISOC}, publisherplace = {Montreal, Kanada} }