engleski

Development of new functional supramolecular systemsbased onβ-cyclodextrin and liposomes for gene delivery

The aim of the present study was preparation and characterization of supramolecularsystems with adamantyl guanidine (AG) and probing of their interaction with DNA usinguorescence correlation spectroscopy (FCS). In the continuation of our study dealing withadamantyl guanidines and investigations of lipid-based nanovesicles as drug delivery systems[1, 2] we prepared and characterized functionalized supramolecular systems of AG 1-5 withphosphatidylcholine liposomes, liposomes incorporating amphiphilicβ- cyclodextrin (β-CD)derivative and cyclodextrin vesicles composed only from the amphiphilicβ-CD derivative.Eciency of incorporation of AG 1-5 into prepared supramolecular systems was measuredspectrophotometrically, and surface charge were determined by dynamic light scatteringmethod. FCS was applied to examine the interactions between functionalized supramolecularsystems and the Cy5 uorescently labelled, double-stranded, 120 bp DNA (DNA120*). Theobtained results have clearly demonstrated that the supramolecular systems with entrappedAG 1-4 present guanidinium groups on the surface of vesicles, which leads to interactionwith DNA120* via guanidine- phosphate interaction. Preliminary results have shown thatprepared functional supramolecular systems strongly bind DNA and as such could be usedin gene delivery.

Adamantyl guanidine ; cyclodextrine ; supramolecular complexes, liposomes, host-guest interaction, fluorescence correlation spectroscopy ; gene delivery

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