engleski

Extraction of bioactive components from tobacco industry waste

Tobacco industry produce a huge amount of waste, which is categorized as agro-industrial waste. The aim of this work was to investigate the influence of different ultrasound extraction conditions on the content of phenolic components and solanesol in tobacco waste extracts. Tobacco wastes: fraction 1 (leaf waste and mid-rib) and fraction 2 (dust) were obtained from tobacco factory Hrvatski duhani, Virovitica. The ultrasound-assisted extraction from tobacco wastes was performed. The influence of extraction temperatures (30, 50, 70 °C), time (15, 30, 45 min), solvent ethanol:water ratio (40%, 60%, 80%)and solvent- solid ratio (10, 30, 50 mL/g)on the phenolic content in obtained extracts were determined. Extraction of solanesol was improved by adding NaOH into the extraction solvent in concentration of 0.05M, under conditions of temperatures (33.78, 40, 55, 70, 76.2 °C) and time (8.78, 15, 30, 45, 51.21 min). Ultrasound extraction conditions had statistically significant influence on content of bioactive components. The content of bioactive components was determined using High Performance Liquid Chromatography (HPLC). Extracts of tobacco waste obtained from fractions 1 were characterized by high levels of chlorogenic acid and rutin with low level of caffeic acid and solanesol. In extracts obtained from fractions 2 low levels of chlorogenic acid, rutin and solanesol were determined.

tobacco waste ; by-product ; ultrasound extraction ; phenolic components ; solanesol

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