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Pregled bibliografske jedinice broj: 962639

Biocompatibility assessment of up- and down-converting nanoparticles: implications of interferences with in vitro assays


(COST Action UPCON (CM1403)) Pem, Barbara; González-Mancebo, Daniel; Moros, Maria; Ocana, Manuel; Becerro, Ana Isabel; Pavičić, Ivan; Selmani, Atidja; Babič, Michal; Horak, Daniel; Vinković Vrček, Ivana
Biocompatibility assessment of up- and down-converting nanoparticles: implications of interferences with in vitro assays // Methods and Applications in Fluorescence, 7 (2019), 1; 014001, 15 doi:10.1088/2050-6120/aae9c8 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 962639 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Biocompatibility assessment of up- and down-converting nanoparticles: implications of interferences with in vitro assays

Autori
Pem, Barbara ; González-Mancebo, Daniel ; Moros, Maria ; Ocana, Manuel ; Becerro, Ana Isabel ; Pavičić, Ivan ; Selmani, Atidja ; Babič, Michal ; Horak, Daniel ; Vinković Vrček, Ivana

Kolaboracija
COST Action UPCON (CM1403)

Izvornik
Methods and Applications in Fluorescence (2050-6120) 7 (2019), 1; 014001, 15

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
photon up- and down-conversion ; nanoparticles ; interferences ; cell viability ; oxidative stress ; biocompatibility ; safety

Sažetak
Safety assessment of nanoparticles (NPs) is a crucial during their design and development for biomedicine. As one of the prerequisite steps during this evaluation is the in vitro testing employing cell-based assays that are not always validated and well-adapted for NPs. Interferences with in vitro assays may arise due to nano-related optical, oxidative, fluorescent, surface and catalytic properties of NPs. Thus, proper validation of each assays system has to be performed for each NPs type. This study aimed to evaluate applicability of the most common in vitro cytotoxicity assays for safety assessment of up- and down-converting lanthanide-doped NPs. Conventional cell viability tests and fluorescence-based assays for oxidative stress response were selected to determine biological effects of up- and down-converting NPs to human brain cells. Comparison was performed with the known silver and iron oxide NPs for verification purpose. Both plate reader and flow cytometry measurements were examined. Obtained results indicated that both types of Ln-doped NPs interfered to much lesser extent than metallic NPs. In addition, the great potential of both up- and down-converting NPs for biomedicine has been manifested due to their biocompatibility and low toxicity.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Farmacija



POVEZANOST RADA


Projekti:
HRZZ-IP-2016-06-2436 - Značaj interakcija metalnih nanočestica sa sumpornim biomolekulama za nano-bio sučelje (NanoFaceS) (Vinković Vrček, Ivana, HRZZ - 2016-06) ( POIROT)

Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Institut "Ruđer Bošković", Zagreb

Citiraj ovu publikaciju

(COST Action UPCON (CM1403)) Pem, Barbara; González-Mancebo, Daniel; Moros, Maria; Ocana, Manuel; Becerro, Ana Isabel; Pavičić, Ivan; Selmani, Atidja; Babič, Michal; Horak, Daniel; Vinković Vrček, Ivana
Biocompatibility assessment of up- and down-converting nanoparticles: implications of interferences with in vitro assays // Methods and Applications in Fluorescence, 7 (2019), 1; 014001, 15 doi:10.1088/2050-6120/aae9c8 (međunarodna recenzija, članak, znanstveni)
(COST Action UPCON (CM1403)) (COST Action UPCON (CM1403)) Pem, B., González-Mancebo, D., Moros, M., Ocana, M., Becerro, A., Pavičić, I., Selmani, A., Babič, M., Horak, D. & Vinković Vrček, I. (2019) Biocompatibility assessment of up- and down-converting nanoparticles: implications of interferences with in vitro assays. Methods and Applications in Fluorescence, 7 (1), 014001, 15 doi:10.1088/2050-6120/aae9c8.
@article{article, year = {2019}, pages = {15}, DOI = {10.1088/2050-6120/aae9c8}, chapter = {014001}, keywords = {photon up- and down-conversion, nanoparticles, interferences, cell viability, oxidative stress, biocompatibility, safety}, journal = {Methods and Applications in Fluorescence}, doi = {10.1088/2050-6120/aae9c8}, volume = {7}, number = {1}, issn = {2050-6120}, title = {Biocompatibility assessment of up- and down-converting nanoparticles: implications of interferences with in vitro assays}, keyword = {photon up- and down-conversion, nanoparticles, interferences, cell viability, oxidative stress, biocompatibility, safety}, chapternumber = {014001} }
@article{article, year = {2019}, pages = {15}, DOI = {10.1088/2050-6120/aae9c8}, chapter = {014001}, keywords = {photon up- and down-conversion, nanoparticles, interferences, cell viability, oxidative stress, biocompatibility, safety}, journal = {Methods and Applications in Fluorescence}, doi = {10.1088/2050-6120/aae9c8}, volume = {7}, number = {1}, issn = {2050-6120}, title = {Biocompatibility assessment of up- and down-converting nanoparticles: implications of interferences with in vitro assays}, keyword = {photon up- and down-conversion, nanoparticles, interferences, cell viability, oxidative stress, biocompatibility, safety}, chapternumber = {014001} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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