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Bacterial isolates from crayfish surface biofilm as potential source of anti-omycete compounds (CROSBI ID 667432)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Orlić, Karla ; Šver, Lidija ; Burić, Lucija ; Kazazić, Snježana ; Hrašćan, Reno ; Vladušić, Tomislav ; Bielen, Ana Bacterial isolates from crayfish surface biofilm as potential source of anti-omycete compounds // Programme and Abstracts of 6th Central European Symposium on Antimicrobials and Antimicrobial Resistance CESAR 2018 / Abram, Maja ; Bielen, Ana ; Kifer, Domagoj et al. (ur.). Zagreb: Hrvatsko mikrobiološko društvo, 2018. str. 104-104

Podaci o odgovornosti

Orlić, Karla ; Šver, Lidija ; Burić, Lucija ; Kazazić, Snježana ; Hrašćan, Reno ; Vladušić, Tomislav ; Bielen, Ana

engleski

Bacterial isolates from crayfish surface biofilm as potential source of anti-omycete compounds

Oomycetes (water moulds) are a group of eukaryotic microorganisms that include many pathogens of terrestrial and aquatic species. Among freshwater oomycetes, microorganism Aphanomyces astaci (Schikora, 1906) is a causative agent of lethal disease crayfish plague and a reason for decline of native decapod crayfish populations all over Europe. During the infection, germinating A. astaci zoospores penetrate through the crayfish cuticle, followed by growth of hyphae through the host tissue. However, upon the arrival of zoospores on the crayfish cuticle, they encounter host epibiontic biofilm communities. While the protective role of commensal microbes has been shown in other models, the interaction of A. astaci and host biofilm is completely unexplored. Therefore, the aim of this work was to explore the potential of bacterial isolates originating from crayfish cuticle to inhibit A. astaci growth. We took swabs of cuticle biofilm from five specimens of narrow-clawed crayfish, Astacus leptodactylus Eschscholtz, 1823 and five specimens of signal crayfish, Pacifastacus leniusculus (Dana, 1852) and inoculated them on PG1 medium. Next, we have isolated morphologically different individual colonies from mixed cultures and taxonomically identified each isolate by MALDI–TOF mass spectrometry and 16S rRNA gene sequencing. Dominant bacterial genera among isolates were Pseudomonas and Acinetobacter. Furthermore, we tested the potential of isolates to inhibit the growth of A. astaci mycelium by plate inhibition assay. Out of 46 isolates from narrow-clayed crayfish, 13 exhibited great potential for A. astaci inhibition (28 %), while out of 44 isolates from signal crayfish, only 7 showed great inhibition potential (16 %). Most of the potent inhibitors belonged to genus Pseudomonas, for which inhibition of fish pathogenic oomycete from genus Saprolegnia was previously shown. In conclusion, we have characterized cultivable crayfish-associated biofilm communities and shown their potential as a source of anti- oomycete compounds.

Aphanomyces astaci ; crayfish epibionts ; anti-oomycete activity

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Podaci o prilogu

104-104.

2018.

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objavljeno

978-953-7778-16-3

Podaci o matičnoj publikaciji

Programme and Abstracts of 6th Central European Symposium on Antimicrobials and Antimicrobial Resistance CESAR 2018

Abram, Maja ; Bielen, Ana ; Kifer, Domagoj ; Maravić Vlahoviček, Gordana ; Šegvić Klarić, Maja

Zagreb: Hrvatsko mikrobiološko društvo

Podaci o skupu

6th Central European Symposium on Antimicrobials and Antimicrobial Resistance (CESAR 2018)

poster

19.09.2018-22.09.2018

Sveti Martin na Muri, Hrvatska

Povezanost rada

Biologija, Biotehnologija