engleski

Croatian buckwheat landrace populations and genetic diversity assessment via microsatellite markers

Buckwheat (Fagopyrum esculentum Moench) is an old, gluten free pseudo-cereal originating from Asia whose importance increased in Croatia as well due to healthy diet trends. It is produced as a main or post-crop after winter cereals, with very low inputs which makes it attractive for the farmers. In Croatia it is traditionally grown in the northwestern part – Croatian Zagorje, Podravina and in Međimurje. With increasing interest for buckwheat production and consumption, lack of the adequate reproduction material becomes an issue since in Croatia there is no buckwheat breeding program or seed trade. There is therefore a need for collecting, genotyping and revitalizing the remaining Croatian buckwheat germplasm. Accordingly, the aim of this research was to determine genetic profiles and genetic structure of local buckwheat landrace populations that were collected earlier. Molecular genetic analysis of 12 microsatellite (SSR) markers was done for progeny obtained from reselection (25 individuals per population) as well as for international reference varieties (10 individuals per variety). Low level of intervarietal genetic variability was determined and thus it was hard to clearly separate populations. Due to high intravarietal variability further reselection of chosen populations is needed. Reference varieties were genetically more homogenous as expected.

Buckwheat, local population, landrace, microsatellite, genetic diversity

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