engleski

Retinoic acid dependent reprogramming in neuro- and glioblastoma cell lines

Retinoic acid is a differentiation and therefore a therapeutic agent for a certain blood and solid tumours of neural origin. In general, tumour cell response is greatly dependent on signalling network present in specific cell type. We analysed the response of three tumour cell lines of neuronal origin, on prolonged treatment with retinoic acid in combination with PARP inhibitor. PARP has the ability to take part in chromatin remodelling and its inhibition could have an influence on induced differentiation. Neuroblastoma SH SY5Y cells showed retinoic acid dependent differentiation and PARP inhibition did not influence this process. Neuroglioblastoma H4 cells continued to proliferate and showed signs of epithelial-mesenchymal transition after prolonged treatment with retinoic acid. Glioblastoma A1235 cells also continued to proliferate, increased activity of extracellular protease urokinase plasminogen activator, but did not show other characteristics of epithelial-mesenchymal reprogramming, under the same treatment conditions. The data from different and yet related cell types revealed the complexity of cellular reprogramming processes dependent on the intracellular milieu of the cell.

retinoic acid

nije evidentirano