Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

Biodegradation study of methadone by adapted activated sludge: Elimination kinetics, transformation products and ecotoxicological evaluation (CROSBI ID 255317)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Kostanjevečki, Petra ; Sviličić Petrić, Ines ; Lončar, Jovica ; Smital, Tvrtko ; Ahel, Marijan ; Terzić, Senka Biodegradation study of methadone by adapted activated sludge: Elimination kinetics, transformation products and ecotoxicological evaluation // Chemosphere, 214 (2019), 719-728. doi: 10.1016/j.chemosphere.2018.09.153

Podaci o odgovornosti

Kostanjevečki, Petra ; Sviličić Petrić, Ines ; Lončar, Jovica ; Smital, Tvrtko ; Ahel, Marijan ; Terzić, Senka

engleski

Biodegradation study of methadone by adapted activated sludge: Elimination kinetics, transformation products and ecotoxicological evaluation

The biotransformation study of difficult-to- degrade opioid analgesic methadone (MTHD) was performed by activated sludge culture adapted to high concentration of methadone (10 mg/L). The study included determination of elimination kinetics of the parent compound, taxonomic characterization of microbial culture, identification of biotransformation products (TPs) and assessment of ecotoxicological effects of biotransformation processes. The chemical analyses were performed by ultra- performance liquid chromatography/quadrupole- time-of-flight mass spectrometry, whereas the ecotoxicological assessment was made based on determinations of toxicity to freshwater algae. Changes of the adapted sludge culture during the experiment were followed using the 16S rRNA gene amplicon sequencing. Depending on the experimental conditions, the elimination efficiency of methadone (10 mg/L) varied from 9% to 93% with the corresponding half-lives from 11.4 days to 1.5 days. A significantly faster elimination (t1/2 from 1.5 days to 5.8 days) was achieved at cometabolic conditions, using glucose-containing media, as compared to the experiments with MTHD as a single organic carbon source (t1/2 = 11.4 days). Moreover, increased biotransformation rate following the additional supplementation of ammonia, revealed a possible importance of nitrogen availability for the transformation at cometabolic conditions. The elimination of parent compound was associated with the formation of 3 different TPs, two of which were identical to main human metabolites of MTHD, 2- Ethylidene- 1, 5-dimethyl-3, 3- diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl- 3, 3-diphenyl-1-pyrroline (EMDP). EDDP represented over 90% of the total TP concentration at the end of experiment. The biodegradation of MTHD was associated with a pronounced drop in algal toxicity, confirming a rather positive ecotoxicological outcome of the achieved biotransformation processes.

Methadone ; Biodegradation ; Biotransformation ; Transformation products ; EDDP ; Ecotoxicological evaluation

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

214

2019.

719-728

objavljeno

0045-6535

1879-1298

10.1016/j.chemosphere.2018.09.153

Povezanost rada

Interdisciplinarne prirodne znanosti

Poveznice
Indeksiranost