hrvatski

Tradescantia test za procjenu genotoksičnosti atmosferskih polutanata

Pollution of the environment has become one of significant problems of today, especially air pollution due to the negative impact of air pollutants on health of people, animals and plants. Several test have been developed in order to examine and assess the impact of air pollution on the quality of life, such as Tradescantia micronucleus test (Trad-MCN) which uses plants and clones of the Tradescantia genus as model organisms. Trad-MCN test has been developed to study the effect of the gaseous mutagens, and nowadays is one of the most often used plant toxicity tests in studies of genotoxic impact of various mutagenic substances related to soil pollution, heavy metals, traffic gas emission, waste water, petrochemical gas emission, smog, etc. The aim of our study was to give an overview of the advantages and disadvantages of Trad-MCN test, and to determine whether the test should be further improved. Data base composed of results of up to now conducted studies with Trad-MCN test allows further research of the degree of genotoxicity in specific environmental situations (studies of combined effect of various specific substances), comparison of obtained results, and design of further research and strategic defence against the negative impact of pollutants. Research on heavy metals show strong positive reaction of potential carcinogenic metals with Trad-MCN test, while non-carcinogenic metals don’t show such reaction. Results of additional research indicate that the test can be used in monitoring of the effect of heavy metals, pesticides, radionuclides, and other substances. The limitation of the test is in its inapplicability in the studies of specific substances for which cooperation of several enzymes is necessary, thus in future research a combination of plant toxicity tests with other available tests will be necessary. Such approach will result in higher quality of the ecological studies, and will give a more complete characterisation of the environment condition.

Tradescantia test ; onečišćenje zraka ; genotoksičnost

nije evidentirano

engleski

Tradescantia test for the assessment of the genotoxicity of the atmospheric pollutants

Pollution of the environment has become one of significant problems of today, especially air pollution due to the negative impact of air pollutants on health of people, animals and plants. Several test have been developed in order to examine and assess the impact of air pollution on the quality of life, such as Tradescantia micronucleus test (Trad-MCN) which uses plants and clones of the Tradescantia genus as model organisms. Trad-MCN test has been developed to study the effect of the gaseous mutagens, and nowadays is one of the most often used plant toxicity tests in studies of genotoxic impact of various mutagenic substances related to soil pollution, heavy metals, traffic gas emission, waste water, petrochemical gas emission, smog, etc. The aim of our study was to give an overview of the advantages and disadvantages of Trad-MCN test, and to determine whether the test should be further improved. Data base composed of results of up to now conducted studies with Trad-MCN test allows further research of the degree of genotoxicity in specific environmental situations (studies of combined effect of various specific substances), comparison of obtained results, and design of further research and strategic defence against the negative impact of pollutants. Research on heavy metals show strong positive reaction of potential carcinogenic metals with Trad-MCN test, while non-carcinogenic metals don’t show such reaction. Results of additional research indicate that the test can be used in monitoring of the effect of heavy metals, pesticides, radionuclides, and other substances. The limitation of the test is in its inapplicability in the studies of specific substances for which cooperation of several enzymes is necessary, thus in future research a combination of plant toxicity tests with other available tests will be necessary. Such approach will result in higher quality of the ecological studies, and will give a more complete characterisation of the environment condition.

Tradescantia test ; air pollution ; genotoxicity

nije evidentirano