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Pregled bibliografske jedinice broj: 959709

Characterisation of the integrin alpha v-dependent adhesome in mda-mb-435s melanoma cells


Paradžik, Mladen; Humphries, Jonathan D.; Nestić, Davor; Majhen, Dragomira; Dekanić, Ana; Stojanović, Nikolina; Sedda, Delphine; Weber, Igor; Humphries, Martin J.; Ambriović- Ristov, Andreja
Characterisation of the integrin alpha v-dependent adhesome in mda-mb-435s melanoma cells // Abstracts of the 25th Biennial Congress of the European Association for Cancer Research, in ESMO Open, Volume 3, Supplement 2 / Berns, Anton (ur.).
London: BMJ Publishing Group Limited, 2018. str. A100-A100 doi:10.1136/esmoopen-2018-EACR25.240 (poster, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 959709 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Characterisation of the integrin alpha v-dependent adhesome in mda-mb-435s melanoma cells

Autori
Paradžik, Mladen ; Humphries, Jonathan D. ; Nestić, Davor ; Majhen, Dragomira ; Dekanić, Ana ; Stojanović, Nikolina ; Sedda, Delphine ; Weber, Igor ; Humphries, Martin J. ; Ambriović- Ristov, Andreja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 25th Biennial Congress of the European Association for Cancer Research, in ESMO Open, Volume 3, Supplement 2 / Berns, Anton - London : BMJ Publishing Group Limited, 2018, A100-A100

Skup
25th Biennial Congress of the European Association for Cancer Research

Mjesto i datum
Amsterdam, Nizozemska, 30.06.-03.07.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Integrins ; IAC composition ; sensitivity ; αV knockdown

Sažetak
Introduction Integrins are heterodimeric glycoproteins that bind cells to extracellular matrix proteins. Upon integrin clustering, multimolecular integrin adhesion complexes (IACs) form that facilitate the linkage between integrins and the actin cytoskeleton and permit bidirectional signalling. The αV integrin is expressed in most tumour cells where it regulates an array of cellular functions and plays a role in anti-tumour drug resistance. The aim of our work was to assess αV-dependent changes in IAC composition in MDA-MB-435S melanoma cells in order to better understand the increased sensitivity to paclitaxel and vincristine upon integrin αV knockdown. Material and methods Integrin αV- specific shRNA was cloned into pSUPER.puro, transfected into MDA-MB-435S cells using Lipofectamine, and cell clones were selected using puromycin. The sensitivity of cells to antitumor drugs was determined using an MTT assay. Cell migration was monitored using a Transwell assay. IACs were isolated following crosslinking and their molecular composition analysed using mass spectrometry (MS)–based proteomics. Results and discussions In two MDA-MB-435S- derived cell clones with decreased expression of integrin αV, expressing 15% (2αV) or 5% (3αV) of the control cells amount, increased sensitivity to paclitaxel and vincristine, decreased sensitivity to cisplatin, and decreased migration were observed in line with previous results obtained following transient transfection with integrin αV siRNA. In cell clones 2αV and 3αV, which were smaller than control cells and lacked stress fibres, the number of focal adhesions was shown to be significantly lower as observed by interference reflection microscopy and immunofluorescence detection of phospho-paxillin, phospho-FAK and phospho-Src. MS analysis of isolated IACs from control MDA-MB- 435S, 2αV and 3αV cells identified 282 proteins, including 36 out of 60 consensus adhesome proteins. As expected, in clones 2αV and 3αV, integrins αV, β3 and β5 were detected at much lower levels compared with control cells. In addition, lower levels of talin-1 and −2, vinculin, alpha-actinin-4, tensin-3, filamin-A and -B, liprin β1 and plectin were detected. Conclusion These data will enable follow-up analyses of the mechanisms of signalling by integrins αVβ3/β5 and therefore represent a valuable resource to improve our understanding of the mechanisms involved in adhesion control of cell sensitivity to antitumor drugs and metastatic potential.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
IP-2013-11-2465 - Molekularni mehanizmi povećanja osjetljivosti na protutumorske lijekove stanica karcinoma dojke i melanoma čovjeka utišavanjem integrina (INSILCELL) (Ambriović Ristov, Andreja, HRZZ - 2013-11) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Poveznice na cjeloviti tekst rada:

doi www.esmoopen.com doi.org

Citiraj ovu publikaciju:

Paradžik, Mladen; Humphries, Jonathan D.; Nestić, Davor; Majhen, Dragomira; Dekanić, Ana; Stojanović, Nikolina; Sedda, Delphine; Weber, Igor; Humphries, Martin J.; Ambriović- Ristov, Andreja
Characterisation of the integrin alpha v-dependent adhesome in mda-mb-435s melanoma cells // Abstracts of the 25th Biennial Congress of the European Association for Cancer Research, in ESMO Open, Volume 3, Supplement 2 / Berns, Anton (ur.).
London: BMJ Publishing Group Limited, 2018. str. A100-A100 doi:10.1136/esmoopen-2018-EACR25.240 (poster, međunarodna recenzija, sažetak, znanstveni)
Paradžik, M., Humphries, J., Nestić, D., Majhen, D., Dekanić, A., Stojanović, N., Sedda, D., Weber, I., Humphries, M. & Ambriović- Ristov, A. (2018) Characterisation of the integrin alpha v-dependent adhesome in mda-mb-435s melanoma cells. U: Berns, A. (ur.)Abstracts of the 25th Biennial Congress of the European Association for Cancer Research, in ESMO Open, Volume 3, Supplement 2 doi:10.1136/esmoopen-2018-EACR25.240.
@article{article, author = {Parad\v{z}ik, Mladen and Humphries, Jonathan D. and Nesti\'{c}, Davor and Majhen, Dragomira and Dekani\'{c}, Ana and Stojanovi\'{c}, Nikolina and Sedda, Delphine and Weber, Igor and Humphries, Martin J. and Ambriovi\'{c}- Ristov, Andreja}, editor = {Berns, A.}, year = {2018}, pages = {A100-A100}, DOI = {10.1136/esmoopen-2018-EACR25.240}, keywords = {Integrins, IAC composition, sensitivity, αV knockdown}, doi = {10.1136/esmoopen-2018-EACR25.240}, title = {Characterisation of the integrin alpha v-dependent adhesome in mda-mb-435s melanoma cells}, keyword = {Integrins, IAC composition, sensitivity, αV knockdown}, publisher = {BMJ Publishing Group Limited}, publisherplace = {Amsterdam, Nizozemska} }
@article{article, author = {Parad\v{z}ik, Mladen and Humphries, Jonathan D. and Nesti\'{c}, Davor and Majhen, Dragomira and Dekani\'{c}, Ana and Stojanovi\'{c}, Nikolina and Sedda, Delphine and Weber, Igor and Humphries, Martin J. and Ambriovi\'{c}- Ristov, Andreja}, editor = {Berns, A.}, year = {2018}, pages = {A100-A100}, DOI = {10.1136/esmoopen-2018-EACR25.240}, keywords = {Integrins, IAC composition, sensitivity, αV knockdown}, doi = {10.1136/esmoopen-2018-EACR25.240}, title = {Characterisation of the integrin alpha v-dependent adhesome in mda-mb-435s melanoma cells}, keyword = {Integrins, IAC composition, sensitivity, αV knockdown}, publisher = {BMJ Publishing Group Limited}, publisherplace = {Amsterdam, Nizozemska} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Emerging Sources Citation Index (ESCI)


Citati:





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