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Influence of extraction mode on the content of phenolics, pigments and antioxidant potential of brown algae extracts

Algae produce wide range of secondary metabolites as a result of adaptation to harsh environmental conditions they grow in. As these metabolites have numerous positive biological activities, algae could be considered as great sources of bioactive compounds. Brown seaweeds are found to contain high amounts of phenolic compounds so the aim of this study was to investigate the potential of two widely spread Adriatic Sea seaweeds ; Cystoseira compressa and Dictyota sp. In order to define the optimal extraction method, the influences of different extraction temperatures (20, 40 and 60ºC) as well as the effect of the used solvents (water, ethanol and acetone) during ultrasound assisted extraction were studied. The extracts were analysed spectrophotometrically for total phenolics, flavonoids, tannins and pigments. C. compressa contained higher amounts of phenolics and flavonoids with the highest amounts detected in acetone extracts, while ethanol was more suitable extraction solvent for Dictyota. Acetone extracts of both species were the richest in tannins. The pigments (chlorophylls, carotenoids) in all extracts were also detected while extracts with the highest content of analysed components were additionally tested for antioxidant activity.

brown seaweeds, Adriatic Sea, total phenolic content, tannins, flavonoids, pigments

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