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Pregled bibliografske jedinice broj: 959473

The inhibitory effect of flavonoid aglycones on the metabolic activity of CYP3A4 enzyme


Šarić Mustapić, Darija; Debeljak, Željko; Maleš, Željan; Bojić, Mirza
The inhibitory effect of flavonoid aglycones on the metabolic activity of CYP3A4 enzyme // Molecules, 23 (2018), 10; 2553, 12 doi:10.3390/molecules23102553 (međunarodna recenzija, članak, znanstveni)


Naslov
The inhibitory effect of flavonoid aglycones on the metabolic activity of CYP3A4 enzyme

Autori
Šarić Mustapić, Darija ; Debeljak, Željko ; Maleš, Željan ; Bojić, Mirza

Izvornik
Molecules (1420-3049) 23 (2018), 10; 2553, 12

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Flavonoids, CYP3A4, HPLC-DAD, testosterone

Sažetak
Flavonoids are natural compounds that have been extensively studied due to their positive effects on human health. There are over 4000 flavonoids found in higher plants and their beneficial effects have been shown in vitro as well as in vivo. However, data on their pharmacokinetics and influence on metabolic enzymes is scarce. The aim of this study was to focus on possible interactions between thirty most commonly encountered flavonoid aglycones on the metabolic activity of CYP3A4 enzyme. 6β- hydroxylation of testosterone was used as marker reaction of CYP3A4 activity. Generated product was determined by HPLC coupled with diode array detector. Metabolism and time dependent, as well as direct inhibition, were tested to determine if inhibition was reversible and/or irreversible. Out of 30 flavonoids tested 7 significantly inhibited CYP3A4, most prominent being acacetin that inhibited 95% of enzyme activity at 1 µM concentration. Apigenin showed reversible inhibition, acacetin and chrysin showed combined irreversible and reversible inhibition while chrysin dimethylether, isorhamnetin, pinocembrin, and tangeretin showed pure irreversible inhibition. These results alert on possible flavonoid-drug interactions on the level of CYP3A4.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Farmacija



POVEZANOST RADA


Projekt / tema
HRZZ-UIP-2014-09-5704 - Metabolizam i interakcije biološki aktivnih spojeva i QSAR (MIRZA BOJIć, )

Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb,
Prirodoslovno-matematički fakultet, Zagreb,
Klinički bolnički centar Osijek,
Medicinski fakultet, Osijek

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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