engleski

Differences in the antioxidative response of three soybean cultivars to cadmium

Anthropogenic activities increased cadmium content in agriculture soils leading to its entry into the food chain, thus causing numerous pathological consequences for the consumers. In addition, cadmium causes changes in physiological and biochemical processes in plants affecting their growth and yield. Different plant species, but also different cultivars of the same species, vary widely in the terms of cadmium accumulation and tolerance. Considering it, production of soybean (Glycine max L.) as a leading oil and protein culture is endangered by low cadmium tolerance. Therefore, the aim of this study is to determine differences in the biochemical mechanisms of cadmium tolerance in leaves of three Croatian soybean cultivars (Korana, Ika and Lucija), which differ in the capacity of cadmium accumulation. As the indicators of oxidative stress, content of H 2 O 2 and lipid peroxidation levels expressed in terms of thiobarbituric acid reactive substances were determined, while antioxidative status was determined by the activities of antioxidative enzymes such as catalase, ascorbate peroxidase, glutathione peroxidase and guaiacol peroxidase. Cadmium treatment increased lipid peroxidation levels in leaves of all three soybean cultivars, indicating presence of oxidative stress. Also, the activity of glutathione peroxidase was increased in all cadmium treated plants, while other enzymes showed different activity patterns in investigated cultivars. Cultivar Korana, that showed the highest level of cadmium accumulation, also demonstrated the highest level of tolerance in regard to other two cultivars. The most sensitive cultivar Ika with moderate accumulation capacity showed the lowest level of antioxidative response. Biochemical mechanisms of tolerance to cadmium stress differed in three investigated soybean cultivars, and tolerance to cadmium was partly achieved by increased antioxidative response.

antioxidative response ; cadmium ; oxidative stress ; soybean ; cultivars

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