engleski

Interaction of Hydrodynamic Environment On Performance of Homogeneous Bioreactors With Enzyme Kinetic Models

In the work is from theoretical and computational point of view analyzed interaction of hydrodynamic effects on enzyme reactions in homogeneous non-ideal industrial bioreactors. The results are extended to the case of unstructured model of a chemostat with an extracellular product synthesis. Considered are the cases of two limiting hydrodynamic states: 1) total segregation or macromixing; 2) complete or micro mixing. The effects of hydrodynamic conditions are represented by assumed experimentally determined residence time distribution curve. For the case of complete molecular mixing Zwietering's life expectancy function is applied. For macromixing state conversions are determined by convolution of residence time distribution function with conversion functions from batch kinetics. Studied are effects of degree of mixing on efficiency of Michaelis-Menten kinetics without inhibition and with substrate inhibition (negative order kinetics). Chemostat is modeled with unstructured Monod based kinetics for biomass growth, substrate consumption and Piret's kinetics for extracellular product synthesis. Theoretical evolution of the residence time distributions is derived from various combinations of perfectly stirred vessels and ideal plug flow in tubes. The aim of the work is to establish practical guidance for estimation of lower and upper bounds for bioconversions in nonideal industrial reactors.

RTD, chemostat, segregated flow

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