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Antitumor Efficacy of Curcumin, Bleomycin and Hyperthermia on Ehrlich Ascites Tumor Cells

Chemotherapy is used to stop proliferation and spreading of cancer cells, as well as to initiate apoptosis in cancer cells. Due to its necrotic nature, chemotherapy usually causes damage to nearby healthy tissue. Flavonoids have shown their capability to fight cancer cells and to prevent some damage to healthy cells. In this research curcumin was tested in vitro for its effect on Ehrlich ascites tumor (EAT) cells, their proliferation and ability to initiate apoptosis/necrosis in the cells, and curcumins effect in interaction with bleomycin and hyperthermia. EAT was grown in Swiss albino mice, the cells were isolated on the 7th day, and were incubated in culture medium, and treated with curcumin, bleomycin and hyperthermia. The antiproliferative effect of the test components was confirmed by a colorimetry assay after a short incubation directly with bleomycin for 2 h and curcumin for 13. HBK 295 13th CBC the next 2 h at physiological or hyperthermal (43°C) conditions and after the treatment of cells for 24 hrs. The percentage of tumor cells in apoptosis and necrosis on the flow cytometer was analyzed and the genotoxic effect of the comet test was investigated. Curcumin demonstrated the potential to inhibit the proliferation of EAT cells in vitro, and to slightly increase the percentage of cells in apoptosis. The combination with cytostatic bleomycin and hyperthermia provides a better anti- proliferative effect which is based on the high percentage induction of necrosis and complete cell lysis.

Ehrlich ascites tumor, bleomycin, curcumin, hyperthermia

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