hrvatski

Morfometrijska analiza i genetska karakterizaicja populacija mediteranske dagnje Mytilus galloprovincialis uzduž hrvatske obale Jadrana

Mussels Mytilus spp. belong to a group of key species in marine coastal ecosystems. This work demonstrated the dominant presence of Mediterranean mussel M. galloprovincialis Lmk. 1819 along the eastern Adriatic coast, from Lim bay to Dubrovnik. Morphological variations based on mussel shell measurements were within normal values for M. galloprovincials (h/l ratio 55.89 ± 5.08%) with several morphotypes determined by outline analysis. However, molecular analyses gave new insight into the genetic diversity of Adriatic mussel M. galloprovincialis. Previously, with the application of nuclear marker Me 15/16 analysis that distinguishes mussels of Mytilus edulis complex species (M. edulis-EE, M. trossulus-TT and M. galloprovincialis-GG), among mussels from all 22 sites, only 2 of 110 specimens were identified as heterozygote GE and GT genotypes, while all others were identified as homozygote GG. The amplified fragment length polymorphism (AFLP) analysis using our new nuclear precollagen-D gene marker (preCol-D) showed that the most prevalent Adriatic M. galloprovincialis preCol–D fragment size is 239/240 bp. Comparing the AFLP results of Adriatic M. galloprovincialis populations with Mytilus spp. (GG, GE, EE, ET and TT) from other locations (Mediterranean, Atlantic, Baltic, southern Pacific) it was possible to distinguish Adriatic-Mediterranean (239/240) and Atlantic (236/237 bp) M. galloprovincialis mussel populations as well as to identify all other species of M. edulis complex.

Oblik ljušture ; Morfotipovi ; Jezgrini biljezi ; Me15/16 ; preCol-D ; AFLP

nije evidentirano

engleski

Morphometric analyses and genetic characterization of Mediterranean mussel Mytilus galloprovincialis populations along the Croatian Adriatic coast

Mussels Mytilus spp. belong to a group of key species in marine coastal ecosystems. This work demonstrated the dominant presence of Mediterranean mussel M. galloprovincialis Lmk. 1819 along the eastern Adriatic coast, from Lim bay to Dubrovnik. Morphological variations based on mussel shell measurements were within normal values for M. galloprovincials (h/l ratio 55.89 ± 5.08%) with several morphotypes determined by outline analysis. However, molecular analyses gave new insight into the genetic diversity of Adriatic mussel M. galloprovincialis. Previously, with the application of nuclear marker Me 15/16 analysis that distinguishes mussels of Mytilus edulis complex species (M. edulis-EE, M. trossulus-TT and M. galloprovincialis-GG), among mussels from all 22 sites, only 2 of 110 specimens were identified as heterozygote GE and GT genotypes, while all others were identified as homozygote GG. The amplified fragment length polymorphism (AFLP) analysis using our new nuclear precollagen-D gene marker (preCol-D) showed that the most prevalent Adriatic M. galloprovincialis preCol–D fragment size is 239/240 bp. Comparing the AFLP results of Adriatic M. galloprovincialis populations with Mytilus spp. (GG, GE, EE, ET and TT) from other locations (Mediterranean, Atlantic, Baltic, southern Pacific) it was possible to distinguish Adriatic-Mediterranean (239/240) and Atlantic (236/237 bp) M. galloprovincialis mussel populations as well as to identify all other species of M. edulis complex.

Shell shape ; Morphotypes ; Nuclear markers ; Me15/16 ; preCol-D ; AFLP

nije evidentirano