engleski

Earthworms coelmocyte extraction - non-invasive method of sample collection for pesticide effect assessment

Earthworms are often used in ecotoxicological research because of natural habitat where they can be exposed to various pesticides. Since a large number of organisms has to be sacrificed it would be useful to develop non-invasive methods for the analysis of target parameters. The aim of this study is to determine whether the coelomocyte extract, obtained by the non- invasive method, can be used to measure responses of biochemical biomarkers and to determine whether it can be used in assessing the effects of pesticides already known to have a negative impact on the earthworms. In the present study Eisenia andrei earthworms were exposed to organophosphate dimethoate by applying the filter paper contact test. After 48 h, coelomocyte extracts were prepared and acetylcholinesterase (AChE) and carboxylesterase (CES) activities were measured. The percentage of inhibition of the measured enzymes in the coelomocyte extract was compared with the inhibition of the same enzymes in the samples obtained from whole body homogenate. The results showed significant inhibition of AChE both in the coelomocyte extract and the whole body homogenate and the inhibition rate was comparable. In the case of CES, the level of inhibition was higher in homogenates, and the high variance in coelomocyte disabled obtaining significant differences. This study indicates the possibility of using the coelomocyte extract for measurement of biochemical biomarkers and assessment of the pesticide effects.

earthworms ; coelomocyte ; pesticides ; biochemical biomarkers

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