Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 955514

The mitotic spindle is chiral due to torques within microtubule bundles


Novak, Maja; Polak, Bruno; Simunić, Juraj; Boban, Zvonimir; Kuzmić, Barbara; Thomae, Andreas W; Tolić, Iva Marija; Pavin, Nenad
The mitotic spindle is chiral due to torques within microtubule bundles // Nature Communications, 9 (2018), 3571, 10 doi:10.1038/s41467-018-06005-7 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 955514 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
The mitotic spindle is chiral due to torques within microtubule bundles

Autori
Novak, Maja ; Polak, Bruno ; Simunić, Juraj ; Boban, Zvonimir ; Kuzmić, Barbara ; Thomae, Andreas W ; Tolić, Iva Marija ; Pavin, Nenad

Izvornik
Nature Communications (2041-1723) 9 (2018); 3571, 10

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
mitosis ; mitotic spindle ; microtubules ; chirality ; torque ; kinesin-5

Sažetak
Mitosis relies on forces generated in the spindle, a micro- machine composed of microtubules and associated proteins. Forces are required for the congression of chromosomes to the metaphase plate and their separation in anaphase. However, besides forces, torques may exist in the spindle, yet they have not been investigated. Here we show that the spindle is chiral. Chirality is evident from the finding that microtubule bundles in human spindles follow a left-handed helical path, which cannot be explained by forces but rather by torques. Kinesin-5 (Kif11/Eg5) inactivation abolishes spindle chirality. Our theoretical model predicts that bending and twisting moments may generate curved shapes of bundles. We found that bundles turn by about −2 deg µm−1 around the spindle axis, which we explain by a twisting moment of roughly −10 pNµm. We conclude that torques, in addition to forces, exist in the spindle and determine its chiral architecture.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija



POVEZANOST RADA


Ustanove
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Maja Novak (autor)

Avatar Url Nenad Pavin (autor)

Avatar Url Iva Marija Tolić (autor)

Avatar Url Barbara Kuzmić (autor)

Avatar Url Juraj Simunić (autor)

Citiraj ovu publikaciju

Novak, Maja; Polak, Bruno; Simunić, Juraj; Boban, Zvonimir; Kuzmić, Barbara; Thomae, Andreas W; Tolić, Iva Marija; Pavin, Nenad
The mitotic spindle is chiral due to torques within microtubule bundles // Nature Communications, 9 (2018), 3571, 10 doi:10.1038/s41467-018-06005-7 (međunarodna recenzija, članak, znanstveni)
Novak, M., Polak, B., Simunić, J., Boban, Z., Kuzmić, B., Thomae, A., Tolić, I. & Pavin, N. (2018) The mitotic spindle is chiral due to torques within microtubule bundles. Nature Communications, 9, 3571, 10 doi:10.1038/s41467-018-06005-7.
@article{article, year = {2018}, pages = {10}, DOI = {10.1038/s41467-018-06005-7}, chapter = {3571}, keywords = {mitosis, mitotic spindle, microtubules, chirality, torque, kinesin-5}, journal = {Nature Communications}, doi = {10.1038/s41467-018-06005-7}, volume = {9}, issn = {2041-1723}, title = {The mitotic spindle is chiral due to torques within microtubule bundles}, keyword = {mitosis, mitotic spindle, microtubules, chirality, torque, kinesin-5}, chapternumber = {3571} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font