engleski

Effects of low-temperature stress on specialized metabolites level in Brassica oleracea var. acephala

Among the various abiotic stresses, low temperature is one of the major environmental constraints that limit the plant development and crop productivity. In the present work, we investigate the responses of kale (Brassica oleracea L. var. acephala), naturally cold-tolerant plants, to low temperatures because we expect that those plants have molecular mechanisms that enable them to effectively cope with low temperatures. Particularly, we were focused on specialized metabolites level because the role of specialized metabolites in low temperature stress tolerance is poorly understood. In a laboratory conditions plants at different growing stage- seedling, and 6-10 week old plants were exposed to chilling (8⁰C) and freezing (-8⁰C) temperature and changes in physiological, molecular and biochemical responses were evaluated in comparison with the control (21⁰C). Among other parameters, the level of the main group of specialized metabolites- glucosinolates, polyphenols and carotenoids were evaluated by UV-VIS and/or HPLC-PDA. Low temperature stresses significantly reduce growth and development of the plants and affect specialized metabolites level.

kale ; specialized metabolites

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