engleski

Dog mammary tumors phospholipids fatty acid profile: possible implications for tumor grading and prognosis

Introduction: During cancer pathogenesis, the lipid metabolism of cells changes radically and therefore alterations in lipid metabolism has been accepted as a major metabolic pathway feature of solid tumors. Attempts to understand the role of different lipids in cancer physiology depend on the ability to accurately monitor alterations in lipid composition at cell level due to the diversity of lipid classes. Mammary gland tumor is a common tumor in pet veterinary medicine. We investigated the differences in total and phospholipid fatty acid composition of tumor and normal tissue sample in attempt to get better insight into the lipid metabolism of tumor cells as well as to improve grading of studied tumors. Methods: Paired samples of tumor and adjacent non-tumor tissue were isolated 37 mammary tumors. After the pathohistological examination of tumor and non-tumor samples, fatty acid profile was determined by GC-MS. Results: The mammary tumor tissue showed significant difference compared to the normal tissue. The most significant differences were the increased content in C20:4n6, the increased and the increased content of 18:1n7, 20:3n6, C22:4n6 and C22:5n6. Conclusions: Observed results showed significant variations in fatty acid profile between tumor and non-tumor tissue of different lipid classes.

Mammary tumor, Fatty acids, Tumor grading, Dogs

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