engleski

The in situ hydroxyapatite tunes the osteogenic efficacy of porous chitosan structure

In situ highly-porous chitosan/hydroxyapatite (CS/HA) biocomposite scaffolds prepared by freeze-gelation technique represents suitable structure for tissue engineering application. Different content of in situ synthesized hydroxyapatite within chitosan shown different microstructure and topography regarding to the HA content which influences pysical and biological properties. The impact of a different scaffold’s composition was investigated on the human mesenchymal stem cells (hMSCs) differentiation performed in a U-CUP bioreactor under perfusion conditioning. The histological and immunohistochemical analysis of cultured bony tissues, and the evaluation of osteogenic genes’ expression indicate that the lower fraction of in situ formed hydroxyapatite in the range of 10–30% within chitosan scaffold could be preferable for bone-construct development. However, chitosan-based scaffolds need to be modified in terms of mechanical performance. Positive modification of 3D-printed macroporous poly(lactic acid) construct has improved the compressive strength of composite hydrogel, confirmed by Young’s modulus ranging from lower values reported for cancellous bone in dry state

chitosan ; hydroxyapatite, osteogenesis, mechanical properties

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